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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 3

sp. nov., a novel marine hydrocarbonoclastic bacterium isolated from surface seawater No Access

Abstract

A novel -related strain, designated 6-D-6, was isolated from the surface seawater collected around Xiamen Island. The novel strain is Gram-stain-negative, rod-shaped and motile, and grows at 10–45 °C, pH 6.0–9.0 and in the presence of 0.5–15.0 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that it belongs to the genus , with the highest sequence similarity to B5 (99.9 %), followed by JC109 (99.5 %), MACL04 (99.3 %) and other 13 species of the genus (93.8 %–95.6 %). The digital DNA–DNA hybridization and average nucleotide identity values between strain 6-D-6 and three close type strains were 40.1–42.9/90.6–91.4 %, and others were below 22.9/85.1 %, respectively. The novel strain contained major cellular fatty acids of C (31.0 %), C ω8 cyclo (23.5 %), C cyclo (9.7 %), C 3OH (8.6 %), summed feature 8 (7.6 %) and C (5.4 %). The genomic G+C content of strain 6-D-6 was 61.38 %. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified phospholipids and one amino-group-containing phospholipid were detected. On the basis of phenotypic and genotypic characteristics, strain 6-D-6 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 6-D-6 (=MCCC 1A01359=KCTC 92480).

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  • Accepted:
  • Published Online:
Keyword(s): Alcanivorax xiamenensis , hydrocarbon degradation , seawater and taxonomy
Funding
This study was supported by the:
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award NIMR-2021-9)
    • Principle Award Recipient: ZongzeShao
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: QiliangLai
  • National Natural Science Foundation of China (Award No. 42030412 and 41976107)
    • Principle Award Recipient: ChunmingDong
  • the Scientific Research Foundation of Third Institute of Oceanography, MNR (Award No. 2019019, 2019021 and 2020009)
    • Principle Award Recipient: XiaotengFu
  • National Natural Science Foundation of China (Award No. 91851203)
    • Principle Award Recipient: ZongzeShao
© 2023 The Authors
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2023-03-31
2025-01-18
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References

  1. Yakimov MM, Golyshin PN, Lang S, Moore ER, Abraham WR et al. Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Int J Syst Bacteriol 1998; 48 Pt 2:339–348 [View Article]
     [Google Scholar]
  2. Fernández-Martínez J, Pujalte MJ, García-Martínez J, Mata M, Garay E et al. Description of Alcanivorax venustensis sp. nov. and reclassification of Fundibacter jadensis DSM 1 21 78T (Bruns and Berthe-Corti 1999) as Alcanivorax jadensis comb. nov., members of the emended genus Alcanivorax. . Int J Syst Evol Microbiol 2003; 53:331–338 [View Article]
     [Google Scholar]
  3. Rivas R, García-Fraile P, Peix A, Mateos PF, Martínez-Molina E et al. Alcanivorax balearicus sp. nov., isolated from Lake Martel. Int J Syst Evol Microbiol 2007; 57:1331–1335 [View Article] [PubMed]
     [Google Scholar]
  4. Wang W, Wang L, Shao Z. Diversity and abundance of oil-degrading bacteria and alkane hydroxylase (alkB) genes in the subtropical seawater of Xiamen Island. Microb Ecol 2010; 60:429–439 [View Article] [PubMed]
     [Google Scholar]
  5. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article] [PubMed]
     [Google Scholar]
  6. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
     [Google Scholar]
  7. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article] [PubMed]
     [Google Scholar]
  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
     [Google Scholar]
  9. Rzhetsky A, Nei M. Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 1992; 35:367–375 [View Article] [PubMed]
     [Google Scholar]
  10. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article] [PubMed]
     [Google Scholar]
  11. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article]
     [Google Scholar]
  12. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
     [Google Scholar]
  13. Na SI, Kim YO, Yoon SH, Ha SM, Baek I et al. UBCG: up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction. J Microbiol 2018; 56:280–285
     [Google Scholar]
  14. Dong X, Cai M. Determinative Manual for Routine Bacteriology Beijing: Scientific Press; 2001
     [Google Scholar]
  15. Liu C, Shao Z. Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 2005; 55:1181–1186 [View Article]
     [Google Scholar]
  16. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. In MIDI Technical Note vol 101 North Newark, Del, USA: MIDI Inc; 1990
     [Google Scholar]
  17. Kates M. Techniques of lipidology:Isolation, Analysis, and Identification of Lipids, 2nd. edn Amsterdam: Elsevier; 1986
     [Google Scholar]
  18. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464
     [Google Scholar]
  19. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci 2009; 106:19126–19131 [View Article]
     [Google Scholar]
  20. Liu C, Wang W, Wu Y, Zhou Z, Lai Q et al. Multiple alkane hydroxylase systems in a marine alkane degrader, Alcanivorax dieselolei B-5. Environ Microbiol 2011; 13:1168–1178 [View Article] [PubMed]
     [Google Scholar]
  21. Liu J, Ren Q, Zhang Y, Li Y, Tian X et al. Alcanivorax profundi sp. nov., isolated from deep seawater of the Mariana Trench. Int J Syst Evol Microbiol 2019; 69:371–376 [View Article] [PubMed]
     [Google Scholar]
  22. Lai Q, Zhou Z, Li G, Li G, Shao Z. Alcanivorax nanhaiticus sp. nov., isolated from deep sea sediment. Int J Syst Evol Microbiol 2016; 66:3651–3655 [View Article] [PubMed]
     [Google Scholar]
  23. Dong C, Lai Q, Liu X, Gu L, Zhang Y et al. Alcanivorax profundimaris sp. nov., a novel marine hydrocarbonoclastic bacterium isolated from seawater and deep-sea sediment. Curr Microbiol 2021; 78:1053–1060 [View Article]
     [Google Scholar]
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Alcanivorax xiamenensis sp. nov., a novel marine hydrocarbonoclastic bacterium isolated from surface seawater
Int J Syst Evol Microbiol 73, 005798 (2023); https://doi.org/10.1099/ijsem.0.005798
/content/journal/ijsem/10.1099/ijsem.0.005798
/content/journal/ijsem/10.1099/ijsem.0.005798
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