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Volume 73, Issue 3

gen. nov., sp. nov., a novel bacterium isolated from oak forest soil No Access

Abstract

A novel bacterial strain, designated BS-T2-15, isolated from forest soil in close proximity to decaying oak wood, was characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences as well as phylogenomic analyses based on coding sequences of 340 concatenated core proteins indicated that strain BS-T2-15 forms a distinct and robust lineage in the branch of the order . The amino acid identity and the percentage of conserved proteins between the genome of strain BS-T2-15 and genomes of closely related type strains ranged from 64.27 to 66.57% and from 40.89 to 49.27 %, respectively, providing genomic evidence that strain BS-T2-15 represents a new genus. Its cells are Gram-stain-negative, aerobic, motile by a polar flagellum, rod-shaped and form incrusted white to ivory colonies. Optimal growth is observed at 20–22 °C, pH 6 and 0% NaCl. The predominant fatty acids of strain BS-T2-15 are C ω7, C and C 2-OH. Its polar lipid profile consists of a mixture of phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol and its main respiratory quinone is ubiquinone 8. The estimated size of its genome is 6.28 Mb with a DNA G+C content of 69.56 mol%. Therefore, on the basis of phenotypic and genotypic properties, the new strain BS-T2-15 represents a novel genus and species for which the name gen. nov., sp. nov., is proposed. The type strain is BS-T2-15 (DSM 113115=UBOCC-M-3373).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Betaproteobacteria , Burkholderiales , Comamonadaceae , oak forest soil and Scleromatobacter humisilvae
Funding
This study was supported by the:
  • Université de Bretagne Occidentale
    • Principle Award Recipient: SophieMieszkin
© 2023 The Authors
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2023-03-08
2024-05-07
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Scleromatobacter humisilvae gen. nov., sp. nov., a novel bacterium isolated from oak forest soil
Int J Syst Evol Microbiol 73, 005793 (2023); https://doi.org/10.1099/ijsem.0.005793
/content/journal/ijsem/10.1099/ijsem.0.005793
/content/journal/ijsem/10.1099/ijsem.0.005793
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