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Volume 73, Issue 3

sp. nov., a novel carotenoid-producing bacterium isolated from coastal sediment No Access

Abstract

A Gram-stain-negative, aerobic, non-motile and pleomorphic bacterium designated as YG55 was isolated from a coastal sediment sample. Growth was found to occur at 10–37 °C (optimum, 28 °C), at pH 6–9 (optimum, pH 8) and in 0–6 % NaCl (optimum, 1 %). The results of 16S rRNA gene-based analysis showed that strain YG55 was related to the members of the genus and shared the highest identity of 99.4 % with GDMCC 1.2307, followed by JCM 17037 (98.4 %). The phylogenomic results indicated that strain YG55 formed an independent branch distinct from the reference type strains. The 22.7 and 21.8 % digital DNA–DNA hybridization (dDDH) values and 83.0 and 81.8 % average nucleotide identity (ANI) values between strain YG55 and the two relatives were below the species definition thresholds of 70 % (dDDH) and 95–96 % (ANI), indicating that the strain represents a novel genospecies. The results of chemotaxonomic characterization indicated that the major cellular fatty acids of strain YG55 were summed feature 8 (C 6 and/or C 7), C 2OH and C; the main polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; the respiratory quinone was ubiquinone-10. The genomic size and DNA G+C contents were 3.03 Mbp and 66.98 %. The strain contained carotenoid biosynthesis genes and could produce carotenoids. Based on its genotypic and phenotypic characteristics, strain YG55 is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YG55 (=GDMCC 1.2590 =KCTC 82812).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome analysis , novel species , polyphasic taxonomy and Tsuneonella litorea
Funding
This study was supported by the:
  • GDAS’ Project of Science and Technology Development (Award 2021GDASYL-20210103020)
    • Principle Award Recipient: XiaoqinDeng
  • Science and Technology Program of Guangdong Province (Award 2021B1212050022)
    • Principle Award Recipient: HonghuiZhu
  • Key-Area Research and Development Program of Guangdong Province (Award 2020B0202080005)
    • Principle Award Recipient: HonghuiZhu
© 2023 The Authors
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2023-03-31
2024-05-10
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Tsuneonella litorea sp. nov., a novel carotenoid-producing bacterium isolated from coastal sediment
Int J Syst Evol Microbiol 73, 005785 (2023); https://doi.org/10.1099/ijsem.0.005785
/content/journal/ijsem/10.1099/ijsem.0.005785
/content/journal/ijsem/10.1099/ijsem.0.005785
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