1887

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Volume 73, Issue 3

sp. nov., isolated from shore soil of saline lake in Tibet of China and its genome mining of secondary metabolite Open Access

Abstract

Five aerobic, Gram-stain-negative, non-motile, non-spore-forming, short rod bacteria strains, designated as C3-1-R+6, C3-2-M9, B3-2-R-7, B3-2-R-21 and C3-2-M2, were isolated from shore soil of LungmuCo Lake in Tibet of China. The 16S rRNA gene sequence comparisons confirmed their affiliation to the genus of the family , and showed that they were most closely related to KACC 18788 with 94.26 % similarities. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA–DNA hybridization (dDDH) values between them and the validly published species were all below the thresholds for delineating species, supporting that they were novel species of genus . The ANI, AAI and dDDH values between strains C3-1-R+6 and KACC 18788 were 72, 75, and 18% respectively. Meanwhile, the ANI/AAI and dDDH values between these five isolates were higher than the threshold values, showing that they belonged to the same species of . According to genome comparison, the novel isolates have some special biosynthetic gene clusters of secondary metabolites including bacteriton, aryl-polyene, lantipeptide and t1pks, which were absent from their most related phylogenetic neighbours KACC 18788 and CGMCC 1.12195. The main polar lipids contained phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid, one unidentified glycolipid and five unidentified lipids. The predominant respiratory quinone was MK-7. The major cellular fatty acids were iso-C, summed feature 3 (C 7 and/or C 6) and iso-C 3-OH. The genome size of strain C3-1-R+6 was 5 984 948 bp, and its genomic DNA G+C content was 46.21 mol%. To sum up, the five strains were identified as a novel species of the genus , for which the name sp. nov. was proposed. The type strain was C3-1-R+6 (=CGMCC 1.19194=KCTC 92150).

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  • Accepted:
  • Published Online:
Keyword(s): Parapedobacter , salt lake , secondary metabolite , Sphingobacteriaceae and Tibet
Funding
This study was supported by the:
  • the Strategic Biological Resources Capacity Building Project of Chinese Academy of Sciences (Award KFJ-BRP-017-73)
    • Principle Award Recipient: Ai-HuaLi
  • the National Natural Science Foundation of China (Award 31970004)
    • Principle Award Recipient: Ai-HuaLi
© 2023 Institute of Microbiology, Chinese Academy of Sciences retain copyright
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-03-31
2024-04-28
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Parapedobacter tibetensis sp. nov., isolated from shore soil of saline lake in Tibet of China and its genome mining of secondary metabolite
Int J Syst Evol Microbiol 73, 005784 (2023); https://doi.org/10.1099/ijsem.0.005784
/content/journal/ijsem/10.1099/ijsem.0.005784
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