1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 3

sp. nov. isolated from seashore sand No Access

Abstract

A Gram-stain-positive, non-motile, rod-shaped, facultatively anaerobic bacterium, designated as IB182487, was isolated from a seashore sand sample collected from Zhaoshu Island, PR China. Strain IB182487 grew at pH 6.0–10.0 (optimum, pH 8.0), 4–45 °C (optimum, 25–30 °C) and with 0–17 % (w/v) NaCl (optimum, 2–10 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IB182487 belonged to the genus and was closely related to SMC 4352-2, (96.6 %), LMG 22858 (96.5 %), DSM 17723 (96.3 %) and DSM 25387 (96.1 %). Strain IB182487 had -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan and contained menaquinone MK-7 as the predominant isoprenoid quinone. Its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and three unidentified glycolipids. The major cellular fatty acids of strain IB182487 were iso-C and anteiso-C. The whole genome average nucleotide identity and digital DNA–DNA hybridization analysis between the isolate and its closely related type strains demonstrated that the strain significantly differed from other species. The genomic DNA G+C content of strain IB182487 was 37.4 mol%. On the basis of phenotypic and chemotaxonomic properties, phylogenetic relatedness as well as genomic characteristics, strain IB182487 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is IB182487 (=MCCC 1K04629=JCM 34523).

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Keyword(s): 16S rRNA gene , Metabacillus arenae sp. nov and polyphasic taxonomy
Funding
This study was supported by the:
  • Central Public-interest Scientific Institution Basal Research Fund from Chinese Government (Award 1630052019014)
    • Principle Award Recipient: YonghuaHu
  • Financial Fund of the Ministry of Agriculture and Rural Affairs of China (Award NHYYSWZZZYKZX2020)
    • Principle Award Recipient: YonghuaHu
© 2023 The Authors
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2023-03-31
2024-05-11
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Metabacillus arenae sp. nov., isolated from seashore sand
Int J Syst Evol Microbiol 73, 005783 (2023); https://doi.org/10.1099/ijsem.0.005783
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