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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 6

Isolation and characterization of a novel glycoside hydrolase positive bacterial strain named sp. nov. with ginsenoside-converting activity No Access

Abstract

A novel yellow-pigmented catalase- and oxidase-positive bacterial strain (designated NA20) was isolated from wetland soil and characterized. Results of 16S rRNA and draft genome sequence analysis placed strain NA20 within the genus of the family . Strain NA20 showed ≤97.1 % sequence similarity to members of the genus and the highest sequence similarity was found to DY (97.1%). The draft genome of strain NA20 had a total length of 7 144 125 base pairs. A total of 5659 genes were identified, of which 5613 were CDS and 46 RNA genes were assigned a putative function. Mining the genomes revealed the presence of 225 carbohydrate genes out of 1334 genes. Strain NA20 contained iso-C, iso-C G, iso-C 3-OH and summed feature 3 (C 7 and/or C 6) as major fatty acids. The predominant quinone was MK-7. The major polar lipids were phosphatidylethanolamine, one unknown polar lipid and one unknown aminophospholipid. Additionally, the functional analysis of NA20 showed the conversion of protopanaxatriol-mix type major ginsenosides (Rb1, Rc and Rd) to minor ginsenosides F2 and weak conversion of Rh2 and C-K within 24 h. As a result, the genotypic, phenotypic and taxonomic analyses support the affiliation of NA20 within the genus , for which the name sp. nov. is proposed. The type strain is NA20 (=KACC 22218=LMG 32198).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , bioconversion , draft genome , polyphasic taxonomy and PPD-mix type ginsenosides
Funding
This study was supported by the:
  • This work was supported by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment and by and by Brainpool (BP) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (project no. 2019H1D3A1A02070958).
    • Principle Award Recipient: MuhammadZubair Siddiqi
© 2023 The Authors
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2023-06-08
2024-05-04
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Isolation and characterization of a novel glycoside hydrolase positive bacterial strain named Terrimonas ginsenosidimutans sp. nov. with ginsenoside-converting activity
Int J Syst Evol Microbiol 73, 005781 (2023); https://doi.org/10.1099/ijsem.0.005781
/content/journal/ijsem/10.1099/ijsem.0.005781
/content/journal/ijsem/10.1099/ijsem.0.005781
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