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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 3

sp. nov., a novel endophytic bacterium isolated from bark of No Access

Abstract

A endospore-forming bacterium, designated strain KQZ6P-2, was isolated from surface-sterilized bark of the mangrove plant , collected from Maowei Sea Mangrove Nature Reserve in Guangxi Zhuang Autonomous Region, China. Strain KQZ6P-2 was able to grow at NaCl concentrations in the range of 0–3 % (w/v) with optimum growth at 0–1 % (w/v) NaCl. Growth occurred at 20–42 °C (optimal growth at 30–37 °C) and pH 5.5–6.5 (optimal growth at pH 6.5). The 16S rRNA gene sequence similarity between strain KQZ6P-2 and its closest phylogenetic neighbour JCM 9905 was 98.2 %. Phylogenetic analyses using 16S rRNA gene sequences showed that strain KQZ6P-2 formed a distinct lineage with JCM 9905. The draft genome of strain KQZ6P-2 was 5 937 633 bp in size and its DNA G+C content was 47.2mol%. Comparative genome analysis revealed that the average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values among strain KQZ6P-2 and its related species were below the cut-off levels of 95, 70 and 95.5%, respec-tively. The cell-wall peptidoglycan of strain KQZ6P-2 contained -diaminopimelic acid as the diagnostic diamino acid. Major cellular fatty acids were anteiso-C and C. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, four unidentified phospholipids, an unidentified aminolipid and five unidentified lipids. Based on phylogenetic, phenotypic and chemotaxonomic data, strain KQZ6P-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KQZ6P-2 (=MCCC 1K07172 =JCM 34931).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): endophytic bacterium , novel species , Paenibacillus mangrovi and polyphasic taxonomy
Funding
This study was supported by the:
  • Zunyi Medical University Doctoral Research Funding Project (Award F-988)
    • Principle Award Recipient: Ling-JieMeng
  • Beijing Natural Science Foundation (Award 5224038)
    • Principle Award Recipient: Fei-NaLi
  • National Natural Science Foundation of China (Award 81960642)
    • Principle Award Recipient: LiTuo
© 2023 The Authors
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Paenibacillus mangrovi sp. nov., a novel endophytic bacterium isolated from bark of Kandelia candel
Int J Syst Evol Microbiol 73, 005780 (2023); https://doi.org/10.1099/ijsem.0.005780
/content/journal/ijsem/10.1099/ijsem.0.005780
/content/journal/ijsem/10.1099/ijsem.0.005780
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