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Volume 73, Issue 4

sp. nov., isolated from the root of No Access

Abstract

A novel endophytic actinomycete, designated strain NEAU-365, was isolated from the root of collected from Nanjing, Jiangsu, PR China. Comparative 16S rRNA gene sequencing showed that strain NEAU-365 belonged to the genus but clearly differs from the currently recognized species Z350 (99.31 %), NEAU-KD1 (98.82 %) and MUSC 201 (97.78 %). Phylogenetic tree analysis revealed that strain NEAU-365 clustered with the type strains of the genus . The genome size was 4.1 Mbp with a DNA G+C content of 71.2 mol%. Digital DNA–DNA hybridization and average nucleotide identity values between the genome sequence of strain NEAU-365 and those of Z350(27.5 and 84.0 %), NEAU-KD1(23.4 and 80.9 %) and MUSC 201(20.9 and 77.7 %) were below the recommended thresholds for species delineation. Cells were observed to be irregular cocci shaped. The cell wall contained -diaminopimelic acid and the whole-cell sugars were galactose and rhamnose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid and three unidentified phospholipids. The predominant menaquinone was detected as MK-9(H). The major fatty acids were C, C, C ω9 and 10-methyl C. On the basis of genotypic and phenotypic differences from members of the genus , a novel species, sp. nov. is proposed. The type strain is NEAU-365 (=CCTCC AA 2021033=JCM 35005).

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  • Accepted:
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Keyword(s): Mumia quercus sp. nov. , polyphasic taxonomy and Quercus variabilis
Funding
This study was supported by the:
  • Postdoctoral Start-up Fund of Heilongjiang Province (Award LBH-Q19011)
    • Principle Award Recipient: ChongxiLiu
© 2023 The Authors
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2024-05-14
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Mumia quercus sp. nov., isolated from the root of Quercus variabilis
Int J Syst Evol Microbiol 73, 005776 (2023); https://doi.org/10.1099/ijsem.0.005776
/content/journal/ijsem/10.1099/ijsem.0.005776
/content/journal/ijsem/10.1099/ijsem.0.005776
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