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Volume 73, Issue 3

sp. nov., a marine bacterium isolated from deep seawater No Access

Abstract

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated C281, was isolated from seawater sampled at the Marshallese seamount chain. Results of 16S rRNA gene analysis revealed that strain C281 was most closely related to CZ-AZ5 with 92.7 % sequence similarity. Phylogenetic analysis indicated that the new isolate represented a novel species by forming a distinctive lineage within the family . The DNA G+C content of strain C281 was 38.4 mol%. The genome sizes of strain C281 and the reference strain CZ-AZ5 were 5 962 917 and 5 395 999 bp, respectively. The average nucleotide identity and DNA–DNA hybridization values between strains C281 and CZ-AZ5 were found to be low (69.3 and 17.6 %, respectively). Different functional genes were found in the genome of strain C281, such as , polysaccharide utilization loci and linear azol(in)e-containing peptide cluster coding genes. The NaCl range for growth was 0.5–15.0 %. Positive results were obtained for hydrolysis of Tween 60 and urease. MK-7 was the sole respiratory quinone. The major fatty acids were C 6 and/or C 7, iso-C and iso-C F. The major polar lipids of strain C281 were phosphatidylethanolamine, phosphatidylglycerol, two unidentified lipids and five unidentified glycolipids. On the basis of its taxonomic characteristics, the isolate represents a novel species of the genus , for which the name sp. nov. (type strain C281=KCTC 92171=MCCC M27001) is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacteroidota , Membranihabitans , phylogenetic analysis , seawater and whole-genome sequencing
Funding
This study was supported by the:
  • Scientific Research Project Initiating of Hunan University of Arts and Science (Award E07021003)
    • Principle Award Recipient: GuoLi-Li
  • Natural Science Foundation of China (Award 41876182)
    • Principle Award Recipient: XuXue-Wei
  • China Ocean Mineral Resources Research and Development Association (Award DY135-B2-10)
    • Principle Award Recipient: XuXue-Wei
© 2023 The Authors
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2023-03-30
2024-04-28
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Membranihabitans maritimus sp. nov., a marine bacterium isolated from deep seawater
Int J Syst Evol Microbiol 73, 005774 (2023); https://doi.org/10.1099/ijsem.0.005774
/content/journal/ijsem/10.1099/ijsem.0.005774
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