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Volume 73, Issue 4

sp. nov., isolated from the root of (Linn.) Britt No Access

Abstract

A novel protease-producing actinomycete, designated strain NEAU-ZS1, was isolated from the root of (Linn.) Britt collected from Jiamusi, Heilongjiang, PR China. Comparative 16S rRNA gene sequencing showed that strain NEAU-ZS1 belonged to the genus and was most closely related to ‘’ NEAU-YHS15 (99.2%) and JCM 3291 (99.0%). Phylogenetic tree analysis revealed that strain NEAU-ZS1 formed a monophyletic clade with ‘’ NEAU-YHS15. The genome size was 9.3 Mbp with a DNA G+C content of 70.3 mol%. Digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity values between the genome sequence of strain NEAU-ZS1 and those of ‘’ NEAU-YHS15 (28.6, 83.9 and 79.1 %) and JCM 3291 (18.5, 70.6 and 50.2 %) were below the recommended thresholds for species delineation. The strain formed spherical spore vesicles produced on the aerial hyphae. The cell wall contained -diaminopimelic acid and the whole-cell sugars were glucose and madurose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified glycolipid. The menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major fatty acids were iso-C, C ω5, 10-methy C and C ω7. On the basis of the results of a polyphasic taxonomic study, it is concluded that strain NEAU-ZS1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-ZS1 (=CCTCC AA 2021019= JCM 35655).

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Keyword(s): Perilla frutescens (Linn.) Britt , protease and Sphaerisporangium perillae sp. nov.
© 2023 The Authors
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2023-04-12
2024-05-14
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Sphaerisporangium perillae sp. nov., isolated from the root of Perilla frutescens (Linn.) Britt
Int J Syst Evol Microbiol 73, 005773 (2023); https://doi.org/10.1099/ijsem.0.005773
/content/journal/ijsem/10.1099/ijsem.0.005773
/content/journal/ijsem/10.1099/ijsem.0.005773
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