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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 3

sp. nov, a novel species isolated from soil of in Hunan Province, PR China No Access

Abstract

An actinobacterium strain, designated BH-MK-02, was isolated from the soil of . The taxonomic position was determined using a polyphasic approach. Strain BH-MK-02 grew well on International Project series media and formed well-developed, branched substrate hyphae and aerial mycelium that differentiated into straight spore chains with a wrinkled surface. The diagnostic diamino acid was -diaminopimelic acid. The major menaquinones were MK-9(H), MK-9(H) and MK-9(H). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides, phosphatidylglycerol and unidentified lipid spots. The predominant fatty acids were anteiso-C, iso-C, C and C 7/C 6. The phenotypic characteristics of strain BH-MK-02 indicated that it belonged to the genus . Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain BH-MK-02 was most closely related to CGMCC 4.1833 (99.7 %). However, the average nucleotide identity and digital DNA–DNA hybridization values between the whole-genome sequences of strain BH-MK-02 and CGMCC 4.1833 were 78.1 and 23.2 %, respectively, below the 96.7 and 70 % cut-off points respectively recommended for delineating species. Furthermore, the novel isolate could be distinguished from CGMCC 4.1833 by morphological, physiological and biochemical characteristics. Based on all these data, strain BH-MK-02 (=MCCC 1K06237=JCM 34789) clearly represents a novel species within the genus , for which the name sp. nov. is proposed.

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  • Accepted:
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Keyword(s): average nucleotide identity , digital DNA–DNA hybridization , multilocus sequence analysis , polyphasic taxonomy and Streptomyces
© 2023 The Authors
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2023-03-30
2024-04-28
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Streptomyces longhuiensis sp. nov, a novel species isolated from soil of Lilium brownii in Hunan Province, PR China
Int J Syst Evol Microbiol 73, 005770 (2023); https://doi.org/10.1099/ijsem.0.005770
/content/journal/ijsem/10.1099/ijsem.0.005770
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