1887

Abstract

An actinobacterium strain, designated BH-MK-02, was isolated from the soil of . The taxonomic position was determined using a polyphasic approach. Strain BH-MK-02 grew well on International Project series media and formed well-developed, branched substrate hyphae and aerial mycelium that differentiated into straight spore chains with a wrinkled surface. The diagnostic diamino acid was -diaminopimelic acid. The major menaquinones were MK-9(H), MK-9(H) and MK-9(H). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides, phosphatidylglycerol and unidentified lipid spots. The predominant fatty acids were anteiso-C, iso-C, C and C 7/C 6. The phenotypic characteristics of strain BH-MK-02 indicated that it belonged to the genus . Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain BH-MK-02 was most closely related to CGMCC 4.1833 (99.7 %). However, the average nucleotide identity and digital DNA–DNA hybridization values between the whole-genome sequences of strain BH-MK-02 and CGMCC 4.1833 were 78.1 and 23.2 %, respectively, below the 96.7 and 70 % cut-off points respectively recommended for delineating species. Furthermore, the novel isolate could be distinguished from CGMCC 4.1833 by morphological, physiological and biochemical characteristics. Based on all these data, strain BH-MK-02 (=MCCC 1K06237=JCM 34789) clearly represents a novel species within the genus , for which the name sp. nov. is proposed.

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2023-03-30
2024-04-23
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References

  1. Peng LJ. Lily Resources and Cultivation Kunming: Yunnan Nationalities Publishing House; 2002 pp 10–16
    [Google Scholar]
  2. Haw SG. The Lilies of China B.T. Bastsford; 1986
    [Google Scholar]
  3. Guo TJ, Liu DY. Research progress of wild lily germplasm resources in China. Modern Agricultural Technology 2011; 21:237–246
    [Google Scholar]
  4. Zhao XY. Resources and research situation of the genus Lilium in China. Acta Hortic 1996; 414:59–68 [View Article]
    [Google Scholar]
  5. Roh SM, Jong SL. Proceedings of the intermational symposium on the genus Lilium. . Acta Hort 1994; 414:314–319
    [Google Scholar]
  6. Li S, Li SL, Zhang JZ. Research progress in lily breeding. Acta Horticulture 2006; 33:203–210
    [Google Scholar]
  7. Ji HW, Ding XL. Extraction, separation and preliminary structure identification of lily saponins. Forest Products Chem Ind 2001; 3:47–51
    [Google Scholar]
  8. Hubert B, Drysdale W, William TS. Lilies of the World Their Cultivation and Classification London Country Life Limited Charles Scrivener’s Sons; 1950
    [Google Scholar]
  9. Zhu M, Luo J, Lv H, Kong L. Determination of anti-hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by LC-Q-TOF-MS/MS. Journal of Functional Foods 2014; 6:585–597 [View Article]
    [Google Scholar]
  10. Long YY, Zhang JZ. Protection and utilization of lily plant resources. Plant Res Environ 1998; 7:40–44
    [Google Scholar]
  11. Neng LQ. Study on tissue culture and rapid propagation of Lilium brownii var. viridulum. Acta Agriculturae Jiangxi 2002; 14:43–46
    [Google Scholar]
  12. Ma T, Zhen W, Zhang YM. Bioassay-guided isolation of anti-inflammatory components from the bulbs of Lilium brownii var. viridulum and identifying the underlying mechanism through acting on the NF-κB/MAPKs pathway. Molecules 2017; 22:506 [View Article]
    [Google Scholar]
  13. Hotchandani T, Desgagne-Penix I. Heterocyclic amaryllidaceaealkaloids: biosynthesis and pharmacological applications. Curr Top Med Chem 2017; 17:418–427 [View Article]
    [Google Scholar]
  14. Masand M, Sivakala KK, Menghani E, Thinesh T, Anandham R et al. Biosynthetic potential of bioactive Streptomycetes isolated from arid region of the desert, Rajasthan (India). Front Microbiol 2018; 9:687 [View Article]
    [Google Scholar]
  15. Khan MS, Gao J, Chen X. The endophytic bacteria Bacillus velezensis Lle-9, isolated from Lilium leucanthum harbors antifungal activity and plant growth-promoting effects. Microbiol Biotech 2020; 30:668–680 [View Article]
    [Google Scholar]
  16. Sun YC, Yi XX, Wang LW, Cheng ZL, Gao JL. Isolation and identification of a lily endophytic bacterium Burkholderia sp.FJb-2 and its in vitro antibacterial and growth-promoting effects. China Soil and Fertilizer 2022; 4:229–236
    [Google Scholar]
  17. Wang SH. Symbiotic Effect of Lanzhou Lily and Arbuscular Mycorrhizal Fungi Lanzhou University; 2008
    [Google Scholar]
  18. Li Y. Separation and identification of oligomycins A and C from Streptomyces luteogriseus ECO 00001 and their bioactive properties. Acta Phytophylacica Sinica 2008; 35:47–50
    [Google Scholar]
  19. Li K, Guo Y, Wang J, Wang Z, Zhao J et al. Streptomyces aquilus sp. nov., a novel actinomycete isolated from a Chinese medicinal plant. Int J Syst Evol Microbiol 2020; 70:1912–1917 [View Article] [PubMed]
    [Google Scholar]
  20. Mo P, Yu Y-Z, Zhao J-R, Gao J. Streptomyces xiangtanensis sp. nov., isolated from a manganese-contaminated soil. Antonie van Leeuwenhoek 2017; 110:297–304 [View Article]
    [Google Scholar]
  21. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313–340 [View Article]
    [Google Scholar]
  22. Ridgway R. Color Standards and Color Nomenclature Washington, DC: Published by the author; 1912 pp 1–43
    [Google Scholar]
  23. Jiang CR, Ruan JS. Two new species and a new variety of Ampullarella. Acta Microbiologica Sinica 1982; 22:207–211
    [Google Scholar]
  24. Xu LH, Li WJ, Liu ZH, Jiang CL. Actinomycete Systematic-Principle, Methods and Practice Beijing: Science press; 2007
    [Google Scholar]
  25. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974; 28:226–231 [View Article] [PubMed]
    [Google Scholar]
  26. Lechevalier MP, Lechevalier H. Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Evol Microbiol 1970; 20:435–443 [View Article]
    [Google Scholar]
  27. Komagata K, Suzuki KI. Lipid and cell-wall analysis in bacterial systematics. Method Microbiol 1987; 19:161–207
    [Google Scholar]
  28. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article] [PubMed]
    [Google Scholar]
  29. Kroppenstedt RM. Fatty-acid and menaquinone analysis of actinomycetes and related organisms. In Society for Applied Bacteriology Technical Series: Chemical Methods in Bacterial Systematics Academic Press; 1985 pp 173–199
    [Google Scholar]
  30. MIDI Sherlock Microbial Identification System Operating Manual, Version 6.0 Newark DE: MIDI Inc; 2005
    [Google Scholar]
  31. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
    [Google Scholar]
  32. Kai B, Pascal AV, Santos E. The antismash database version 2.0: a comprehensive resource on secondary metabolite biosynthetic gene clusters. Nucleic Acids Res 2018D1
    [Google Scholar]
  33. Medema MH, Blin K, Cimermancic P, de Jager V, Zakrzewski P et al. antiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences. Nucleic Acids Res 2011; 39:W339–46 [View Article] [PubMed]
    [Google Scholar]
  34. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
    [Google Scholar]
  35. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article] [PubMed]
    [Google Scholar]
  36. Kluge AG, Farris JS. Quantitative phyletics and the evolution ofanurans. Systematic Zoology 1969; 18:1 [View Article]
    [Google Scholar]
  37. Tamura K, Stecher G, Kumar S. Molecular evolutionary genetics analysis version 11. Mol Biol Evol 2021; 38:3022–3027 [View Article]
    [Google Scholar]
  38. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  39. Richter M, Rosselló-Móra R, Oliver Glöckner F, Peplies J. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics 2016; 32:929–931 [View Article] [PubMed]
    [Google Scholar]
  40. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
    [Google Scholar]
  41. Meier-Kolthoff JP, Göker M. TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy. Nat Commun 2019; 10:2182 [View Article] [PubMed]
    [Google Scholar]
  42. Lefort V, Desper R, Gascuel O. FastME 2.0: a comprehensive, accurate, and fast distance-based phylogeny inference program. Mol Biol Evol 2015; 32:2798–2800 [View Article]
    [Google Scholar]
  43. Farris JS. Estimating phylogenetic trees from distance matrices. Am Nat 1972; 106:645–668 [View Article]
    [Google Scholar]
  44. Hu SR, Li KQ, Zhang YF, Wang YF, Gao J et al. New insights into the threshold values of multi-locus sequence analysis, average nucleotide identity and digital DNA-DNA hybridization in delineating Streptomyces species. Front Microbiol 2022; 13:910277 [View Article]
    [Google Scholar]
  45. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci 2009; 106:19126–19131 [View Article]
    [Google Scholar]
  46. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 33:152–155
    [Google Scholar]
  47. Cortés-Albayay C, Dorador C, Schumann P, Andrews B, Asenjo J et al. Streptomyces huasconensis sp. nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano. Int J Syst Evol Microbiol 2019; 69:2315–2322 [View Article] [PubMed]
    [Google Scholar]
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