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Volume 73, Issue 3

sp. nov., a paraffin oil-degrading bacterium isolated from a mixture of oil-based drill cuttings and paddy soil No Access

Abstract

We isolated a paraffin oil-degrading bacterial strain from a mixture of oil-based drill cutting and paddy soil, and characterized the strain using a polyphasic approach. The Gram-positive, aerobic, rod-shaped and non-spore-forming strain (SCAU 2101) grew optimally at 50 °C, pH 7.0 and 0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strain represented a distinct clade in the genus neighbouring LMG 28482 (97.1 %). The genome size and DNA G+C content of the strain were 3 969 430 bp and 63.1 mol%, respectively. Whole genome based phylogenomic analyses showed that the average nucleotide identity and digital DNA–DNA hybridization values between strain SCAU 2101 and LMG 28482 were 77.5 and 21.2 %, respectively. The major respiratory quinone was Q-10. The dominant fatty acids were C cyclo 8 (50.6 %), summed feature 8 (C 7 and/or C 6; 22.5 %) and C (13.8 %). The polar lipids of the strain included phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine and diphosphatidylglycerol. Based on the results, strain SCAU 2101 was considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is SCAU 2101 (= CCTCC AB 2021125=KCTC 92067).

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  • Accepted:
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Keyword(s): Chelativorans petroleitrophicus sp. nov. , oil-based drill cutting , paraffin oil-degradation and phylogenomic analysis
© 2023 The Authors
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2023-03-31
2024-05-10
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Chelativorans petroleitrophicus sp. nov., a paraffin oil-degrading bacterium isolated from a mixture of oil-based drill cuttings and paddy soil
Int J Syst Evol Microbiol 73, 005769 (2023); https://doi.org/10.1099/ijsem.0.005769
/content/journal/ijsem/10.1099/ijsem.0.005769
/content/journal/ijsem/10.1099/ijsem.0.005769
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