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Volume 73, Issue 6

sp. nov., an endophytic actinobacterium isolated from the root tissue of No Access

Abstract

An endophytic actinobacterium, designated strain PLAI 1-29, was isolated from the root tissue of collected from Pathum Thani province, Thailand. Strain PLAI 1-29 was characterized using a polyphasic taxonomic approach. It typically exhibited morphological and chemotaxonomic properties of the genus . Strain PLAI 1-29 produced a spiral spore chain on aerial mycelium and grew at 15–40 °C, pH 6–10 on International Project 2 agar. The maximum NaCl concentration for growth was 9 % (w/v). Cells of strain PLAI 1-29 presented -diaminopimelic acid, arabinose, galactose and ribose. The detected phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The major menaquinones were MK-9(H) and MK-9(H). The major cellular fatty acids were -C, -C and -C. The genome-based taxonomic details revealed the assignment of strain PLAI 1-29 to the genus and exhibited low threshold values for the delineation of a novel species by average nucleotide identity– (84.0%), average amino acid identity (80.0%) and digital DNA–DNA hybridization (27.6%) with its closest type strain, S187. Furthermore, several differential physiological and biochemical characteristics were detected between strain PLAI 1-29 and the closest type strain. Based on the combined phenotypic and genomic features, strain PLAI 1-29 (=TBRC 7645=NBRC 113170) is considered to represent a new species, for which we propose the name sp. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , endophytic actinomycete , Streptomyces , whole-genome analysis and Zingiber montanum
Funding
This study was supported by the:
  • The School of Science, King Mongkut’s Institute of Technology Ladkrabang (Award CW-1-2564-M-001)
    • Principle Award Recipient: RawiratPansomsuay
  • The National Science, Research and Innovation Fund (NSRF) (Award RE-KRIS/FF65/23)
    • Principle Award Recipient: ChittiThawai
© 2023 The Authors
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2023-06-08
2024-05-03
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Streptomyces zingiberis sp. nov., an endophytic actinobacterium isolated from the root tissue of Zingiber montanum
Int J Syst Evol Microbiol 73, 005765 (2023); https://doi.org/10.1099/ijsem.0.005765
/content/journal/ijsem/10.1099/ijsem.0.005765
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