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Volume 73, Issue 3

sp. nov., isolated from riverside soil No Access

Abstract

An actinobacterial strain designated MMS20-HV4-12, displaying a high hydrolytic potential for various substrates, was isolated from a riverside soil sample and characterized by polyphasic taxonomic analysis. Growth occurred at 10–37 °C (optimum, 30°C), with NaCl concentrations of 0–4 % (optimum, 0 %) and at pH 7–9 (optimum, pH 8). MMS20-HV4-12 was catalase-positive, oxidase-negative, rod-shaped and formed creamy white-coloured colonies. Based on the results of 16S rRNA gene sequence analysis, MMS20-HV4-12 was found to be mostly related to the type strains of (98.3 % sequence similarity), (98.1 %) and (98.0 %). MMS20-HV4-12 showed optimal growth on Reaoner's 2A agar, forming white-coloured colonies. The diagnostic polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol, the major fatty acids were iso-C, C ω8 and 10-methyl-C, the major isoprenoid quinone was MK-8(H), the diagnostic cell-wall sugar was galactose, and the cell-wall diamino acid was -diaminopimelic acid. The genome of MMS20-HV4-12 was 4.47 Mbp in size with a G+C content of 72.9 mol%. The genome based analysis indicated low relatedness between MMS20-HV4-12 and all compared species of , as the highest digital DNA–DNA hybridization and the orthologous average nucleotide identity values were 26.8 and 83.8% respectively. Based on genotypic, phenotypic and phylogenomic characterization, MMS20-HV4-12 evidently represents a novel species of genus , for which the name sp. nov. (type strain=MMS20-HV4-12=KCTC 49651=LMG 32360) is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): actinobacterium , hydrolytic potential , Nocardioides okcheonensis and riverside soil
Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2020R1F1A1076440)
    • Principle Award Recipient: SeungBum Kim
  • National Institute of Biological Resources
    • Principle Award Recipient: SeungBum Kim
© 2023 The Authors
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2023-03-20
2024-04-28
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Nocardioides okcheonensis sp. nov., isolated from riverside soil
Int J Syst Evol Microbiol 73, 005755 (2023); https://doi.org/10.1099/ijsem.0.005755
/content/journal/ijsem/10.1099/ijsem.0.005755
/content/journal/ijsem/10.1099/ijsem.0.005755
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