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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 3

sp. nov., a cellulolytic bacterium isolated from the deep-sea off Noma-misaki, Japan Open Access

Abstract

Cells from strain GE09, isolated from an artificially immersed nanofibrous cellulose plate in the deep sea, were Gram-stain-negative, motile, aerobic cells that could grow with cellulose as their only nutrient. Strain GE09 was placed among members of , in the , with Z1, a marine degrader of agar, as the closest relative (97.4 % similarity). The average nucleotide identity and digital DNA–DNA hybridization values between GE09 and Z1 were 72.5 and 21.2 %, respectively. Strain GE09 degraded cellulose, xylan and pectin, but not starch, chitin and agar. The different carbohydrate-active enzymes encoded in the genomes of strain GE09 and Z1 highlights their differences in terms of target energy sources and reflects their isolation environments. The major cellular fatty acids of strain GE09 were C 7, C and C 7. The polar lipid profile showed phosphatidylglycerol and phosphatidylethanolamine. The major respiratory quinone was Q-8. Based on these distinct taxonomic characteristics, strain GE09 represents a new species in the genus , for which we propose the name sp. nov. (type strain GE09=DSM 113420=JCM 35003).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cellulose degradation , Cellvibrionaceae and deep sea
Funding
This study was supported by the:
  • Kyokuto Pharmaceutical Industrial
    • Principle Award Recipient: ShigeruDeguchi
  • Kyokuto Pharmaceutical Industrial
    • Principle Award Recipient: MikikoTsudome
  • Japan Prize Foundation
    • Principle Award Recipient: MikikoTsudome
  • Shimadzu Science Foundation
    • Principle Award Recipient: ShigeruDeguchi
  • Japan Society for the Promotion of Science (Award JP19K15956)
    • Principle Award Recipient: MikakoTachioka
  • Japan Society for the Promotion of Science (Award JP25850127)
    • Principle Award Recipient: MikikoTsudome
  • Japan Society for the Promotion of Science (Award JP25120512)
    • Principle Award Recipient: MikikoTsudome
  • Japan Society for the Promotion of Science (Award JP25120512)
    • Principle Award Recipient: ShigeruDeguchi
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-03-02
2024-05-11
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Marinagarivorans cellulosilyticus sp. nov., a cellulolytic bacterium isolated from the deep-sea off Noma-misaki, Japan
Int J Syst Evol Microbiol 73, 005748 (2023); https://doi.org/10.1099/ijsem.0.005748
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