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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 3

sp. nov., sp. nov., and sp. nov., isolated from soil of the Qinghai-Tibetan Plateau No Access

Abstract

Six novel bacterial strains, designated CY22, CY357, LJ419, LJ53, CY399 and CY107 were isolated from soil samples collected from the Qinghai–Tibetan Plateau, PR China. Cells were aerobic, rod-shaped, yellow-pigmented, catalase- and oxidase-positive, Gram-stain-negative, non-motile and non-spore-forming. All strains were psychrotolerant and could grow at 0 °C. The results of phylogenetic and phylogenomic analyses, based on 16S rRNA gene sequences and core genomic genes, indicated that the three strain pairs (CY22/CY357, LJ419/LJ53 and CY399/CY107) were closely related to members of the genus and clustered tightly with two species with validly published names, 12116 and BZ26. Values of digital DNA–DNA hybridization between genome sequences of the isolates and other strains from the GenBank database in the genus were far below the 70.0 % threshold. The genomic DNA G+C content of these six strains ranged from 45.2 to 45.8 %. The major cellular fatty acids of all six strains were -C and summed feature 3 (comprising C 7 and/or C 6). MK-7 was the only respiratory quinone, and phosphatidylethanolamine was the predominant polar lipid for strains CY22, LJ419 and CY399. On the basis of the phenotypic, phylogenetic and genomic evidence presented, these six strains represent three novel members of the genus , for which the names sp. nov., sp. nov. and sp. nov. are proposed. The type strains are CY22 (= GDMCC 1.3045 = KCTC 92299), LJ419 (= GDMCC 1.2872 = JCM 33794) and CY399 (= GDMCC 1.3052 = KCTC 92306), respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Dyadobacter , phylogenetic analysis , phylogenomic analysis , Qinghai-Tibet Plateau and soil
Funding
This study was supported by the:
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: JianguoXu
  • National Key R&D Program of China (Award 2019YFC1200505)
    • Principle Award Recipient: LiyunLiu
  • National Key R&D Program of China (Award 2019YFC1200501)
    • Principle Award Recipient: JingYang
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005747
2023-03-13
2024-04-27
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Dyadobacter chenhuakuii sp. nov., Dyadobacter chenwenxiniae sp. nov., and Dyadobacter fanqingshengii sp. nov., isolated from soil of the Qinghai-Tibetan Plateau
Int J Syst Evol Microbiol 73, 005747 (2023); https://doi.org/10.1099/ijsem.0.005747
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