1887

Abstract

Two strains were isolated from flowers and insects in Japan, namely NBRC 115686 and NBRC 115687, respectively. Based on sequence analysis of the D1/D2 domain of the 26S large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region and physiological characteristics, these strains were found to represent a novel yeast species of the genus . Considering pairwise sequence similarity, NBRC 115686 and NBRC 115687 differ from the type strain of the most closely related species, NRRL Y-17645, by 65–66 nucleotide substitutions with 12 gaps (11.65–11.83 %) in the D1/D2 domain of the LSU rRNA gene. The novel species differ from the closely related species in some physiological characteristics. For example, compared with JCM 8257, NBRC 115686 and NBRC 115687 assimilated -galactose, and could grow at 35 and 37 °C. Hence, the name sp. nov. is proposed to accommodate this species in the genus . The holotype is NBRC 115686 (ex-type strain JCM 35540=CBS 18008).

Funding
This study was supported by the:
  • JST, ACT-X (Award JPMJAX21B9)
    • Principle Award Recipient: TaisukeSeike
  • Japan Society for the Promotion of Science (Award 21K05342)
    • Principle Award Recipient: TaisukeSeike
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2023-03-08
2024-05-10
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