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Volume 73, Issue 3

sp. nov., isolated from feline oral cavities No Access

Abstract

Three bacterial strains, KC07075, KC07079 and KC07084, were isolated from the oral cavity of cats in 2007 in Japan. These strains were Gram-negative rods, exhibited gliding motility, grew in air with 5 % CO, and showed oxidase activity, but not catalase activity. The 16S rRNA gene sequences of the three strains were 100 % identical. The 16S rRNA gene sequence of strain KC07084 showed 92.1 and 91.9% identity to the type strains of and , respectively, and showed 89.3–91.6% identity to other species. The major cellular fatty acids of strain KC07084 were iso-C (58.4 %) and summed feature 11 (13.1 %). The G+C content of DNA from strain KC07084 was 33.7 mol%, and the genome size was 2.92 Mbp. Strains KC07075, KC07079 and KC07084 showed digital DNA–DNA hybridization values (dDDH) values of 99.9 % and average nucleotide identity (ANI) values of 99.98 % with each other, strain KC07084 had dDDH values of 18.7–28.2 % and ANI values of 67.12–72.30 % to the type strains of other species. All known species of the genus inhabiting the oral cavity of dogs and cats have catalase activity, but the three strains, including type strain KC07084, lacked catalase activity. These results of the phylogenetic analysis of the 16S rRNA gene sequence, biochemical characteristics, and dDDH and ANI values suggest that strain KC07084 represents a novel species. We propose the name sp. nov., with KC07084 as the type strain (=JCM 32682=DSM 107252).

  • Received:
  • Accepted:
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Keyword(s): Capnocytophaga , catalase , feline and oral cavity
Funding
This study was supported by the:
  • Japan Agency for Medical Research and Development (Award JP21fk0108615)
    • Principle Award Recipient: MichioSuzuki
  • Japan Agency for Medical Research and Development (Award JP19fk0108069)
    • Principle Award Recipient: KoichiImaoka
  • Japan Agency for Medical Research and Development (Award JP17fk0108315)
    • Principle Award Recipient: KoichiImaoka
  • Japan Society for the Promotion of Science (Award JP15K08662)
    • Principle Award Recipient: MichioSuzuki
  • Ministry of Health, Labour and Welfare (Award 21HA2006)
    • Principle Award Recipient: KenMaeda
  • Ministry of Health, Labour and Welfare (Award 21HA2001)
    • Principle Award Recipient: MichioSuzuki
  • Ministry of Health, Labour and Welfare (Award H30-Shinkogyousei-Shitei-001)
    • Principle Award Recipient: MichioSuzuki
  • Ministry of Health, Labour and Welfare (Award H20-Shinko-Ippan-041)
    • Principle Award Recipient: KoichiImaoka
© 2023 The Authors
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Capnocytophaga catalasegens sp. nov., isolated from feline oral cavities
Int J Syst Evol Microbiol 73, 005731 (2023); https://doi.org/10.1099/ijsem.0.005731
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