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Volume 73, Issue 3

sp. nov. isolated from seleniferous soil No Access

Abstract

A Gram-negative, aerobic bacterial strain, designated LX-88, was isolated from seleniferous soil in Enshi, Hubei Province, PR China. Strain LX-88oxidized elemental selenium to selenite, and produced carotenoids but not bacteriochlorophyll. The isolate grew optimally at 28 °C, pH 8.0 and with 0.5 % (w/v) NaCl. Phylogenetic analysies of the organism’s 16S rRNA and bacterial core gene set sequences indicated that LX-88 belongs to the genus , and has the highest degree of 16S rRNA gene sequence similarity to MN-1 (97.4 %). The LX-88 genome was 3.4 Mbp and had a G+C content of 63.6 mol%. The average nucleotide identity and digital DNA–DNA hybridization values showed low relatedness (below 95 and 70 %, respectively) between strain LX-88 and other strains in the genus . Ubiquinone-10 was the predominant quinone. The polar lipid profile was dominated by diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, an unidentified aminolipid, an unidentified phospholipid and an unidentified lipid. The major fatty acid was summed feature 8 (C 7 and/or C 6). These physiological and biochemical tests facilitated the differentiation of strain LX-88 from other members of the genus . The results of this multifaceted taxonomic study indicate that strain LX-88 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is LX-88 (=MCCC 1K08007=LMG 32570).

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Keyword(s): Croceibacterium , new species , polyphasic analysis and selenium-oxidizing bacteria
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award No. 31970095)
    • Principle Award Recipient: MingshunLi
© 2023 The Authors
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2023-03-15
2024-05-12
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Croceibacterium selenioxidans sp. nov., isolated from seleniferous soil
Int J Syst Evol Microbiol 73, 005726 (2023); https://doi.org/10.1099/ijsem.0.005726
/content/journal/ijsem/10.1099/ijsem.0.005726
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