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Volume 73, Issue 3

sp. nov., a thermophilic xylanolytic bacterium isolated from compost Open Access

Abstract

We isolated and analysed a Gram-negative, facultatively thermophilic, xylan-degrading bacterium that we designated as strain DA-C8. The strain was isolated from compost from Ishigaki Island, Japan, by enrichment culturing using beech wood xylan as the sole carbon source. The strain showed high xylan degradation ability under anaerobic growth conditions. The isolate grew at 37–60 °C (optimum, 55 °C) and pH 4.0–11.0 (optimum, pH 9.0). As well as xylan, strain DA-C8 could use polysaccharides such as arabinoxylan and galactan as carbon sources. Comparison of 16S rRNA gene sequences indicated that strain DA-C8 was most closely related to LC2-13A (93.9 %) and HSCC596 (93.5 %). In phylogenetic analysis, strain DA-C8 belonged to the same lineage as K13 (92.5 %), but there was less statistical support for branching (70 %). Digital DNA–DNA hybridization, average nucleotide identity values and average amino acid sequence identity between strain DA-C8 and LC2-13A were 21.8, 68.3 and 58.2 %, respectively. Those between strain DA-C8 and K13 were 23.7, 67.7 and 57.6 %, respectively. The whole-genome DNA G+C content of strain DA-C8 was 52.3 mol%. The major cellular fatty acids were C (42.9 %), anteiso-C (20.0 %) and anteiso-C (16.7 %), the major quinone was menaquinone 7, and the major polar lipids were unidentified glycolipids. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, a novel genus is proposed— gen. nov.—for the novel species sp. nov. The type strain is DA-C8 (=JCM 34211=DSM 111723).

  • Received:
  • Accepted:
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Keyword(s): facultative anaerobic microbe , Insulambacter , Insulambacter thermoxylanivorax , thermophilic , xylan and xylan degradation
Funding
This study was supported by the:
  • Science and Technology Research Partnership for Sustainable Development (Award JPMJSA1801)
    • Principle Award Recipient: AkihikoKosugi
  • Exploratory Research for Advanced Technology (Award JPMJER1502)
    • Principle Award Recipient: AkihikoKosugi
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-03-21
2024-05-12
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Insulambacter thermoxylanivorax sp. nov., a thermophilic xylanolytic bacterium isolated from compost
Int J Syst Evol Microbiol 73, 005724 (2023); https://doi.org/10.1099/ijsem.0.005724
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