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Volume 73, Issue 3

sp. nov., isolated from effective nodules of L. in central China No Access

Abstract

Three slow-growing rhizobial strains (WYCCWR 12678, WYCCWR 12774 and WYCCWR 13023) isolated from effective nodules of L. (cultivated peanut) sampled in Zhengyang County, Henan Province, central China were characterized using a polyphasic approach. The three strains were assigned to the genus based on phylogenetic analysis of their 16S rRNA sequences. Phylogenetic analysis based on their concatenated gene sequences placed the strains into a distinct lineage. Whole-genome average nucleotide identity (ANI) values between WYCCWR 13023 and WYCCWR 12774 and WYCCWR 12678 were 99.43 and 99.31% respectively. ANI values between WYCCWR 13023 and the most closely related strains were all below 93 %. The digital DNA–DNA hybridization (dDDH) values between WYCCWR 13023 and ‘’ CCBAU 51670, BR 3351 and CCBAU 51649, the three most closely related type strains, were 50.40, 43.50 and 39.20% respectively. Phenotypic characterization also allowed the differentiation of the novel strains from their most closely related type strains. Based on the genotypic and phenotypic features, we conclude that the three strains represent a novel species for which the name sp. nov. is proposed, with WYCCWR 13023 (=GDMCC 1.3180=HAMBI 3760) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): average nucleotide identity , biological nitrogen fixation , digital DNA–DNA hybridization and rhizobia phylogeny
Funding
This study was supported by the:
  • Instituto Politécnico Nacional, Mexico (Award SIP20200726)
    • Principle Award Recipient: EntaoWang
  • Project for Extramural Scientists of State Key Laboratory of Agrobiotechnology (Award 2021SKLAB6-8)
    • Principle Award Recipient: junjiezhang
© 2023 The Authors

Erratum

An erratum has been published for this content:
Erratum: sp. nov., isolated from effective nodules of L. in central China
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2023-03-23
2024-05-11
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Bradyrhizobium zhengyangense sp. nov., isolated from effective nodules of Arachis hypogaea L. in central China
Int J Syst Evol Microbiol 73, 005723 (2023); https://doi.org/10.1099/ijsem.0.005723
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