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Volume 73, Issue 3

Description of sp. nov., isolated from tissue of the coral No Access

Abstract

Three novel strains, SCSIO 12582, SCSIO 12638 and SCSIO 12817, were isolated from tissue of the coral collected from Hainan province, PR China. The results of phylogenetic analysis based on 16S rRNA genes revealed that these three isolates showed nearly identical 16S rRNA gene sequences (99.86 %−99.93 %), forming a separate monophyletic cluster within the genus and closely related to FA028. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) relatedness values among the three strains were 99.94 %−99.96 % and 100 %, respectively, indicating that these three isolates were affiliated with the same species. Analysis of the 16S rRNA gene sequence revealed that SCSIO 12582 (representative of the novel isolates) exhibited 98.49 % sequence similarity to FA028. The ANI and dDDH values between SCSIO 12582 and FA028 were 74.81 % and 18.90 % respectively. These three isolates were facultatively anaerobic, Gram-stain-negative, rod shaped and catalase- and oxidase-positive. The DNA G+C content of SCSIO 12582 was 45.82 %. The major respiratory quinone was Q-9. The main cellular fatty acids were C, summed feature 3 (Cω7/Cω6) and Cω9. The polar lipids present were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. On the basis of the results of the phylogenetic, chemotaxonomic, phenotypic and genomic analysis, it was evident that isolates SCSIO 12582, SCSIO 12638 and SCSIO 12817 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SCSIO 12582 (=JCM35228=GDMCC1.3061).

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Keyword(s): Alkalimarinus corallisp. nov , coral tissue and polyphasic taxonomy
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41890853)
    • Principle Award Recipient: SiZhang
  • K. C. Wong Education Foundation (Award GJTD-2020-12)
    • Principle Award Recipient: JieLi
  • Key Science and Technology Project of Hainan Province (Award ZDKJ202018)
    • Principle Award Recipient: JieLi
© 2023 The Authors
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2023-03-09
2024-05-11
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Description of Alkalimarinus coralli sp. nov., isolated from tissue of the coral Pocillopora damicornis
Int J Syst Evol Microbiol 73, 005722 (2023); https://doi.org/10.1099/ijsem.0.005722
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