Full text loading...
Abstract
A Gram-stain-negative strain, designated BR102T, isolated from a soil sample in Brazil was characterized by a polyphasic approach. Comparative 16S rRNA gene sequences indicated that strain BR102T belonged to the genus Citrobacter . The recN- and whole-genome-based phylogeny, and multilocus sequence analysis based on concatenated partial fusA, leuS, pyrG and rpoB sequences strongly supported a clade encompassing strain BR102T and a strain from public database that was distinct from currently recognized species of the genus Citrobacter . Average nucleotide identity and digital DNA–DNA hybridization values between strain BR102T and the closest relative Citrobacter freundii ATCC 8090T were 91.8 and 48.8 %, respectively. The ability to metabolize different compounds further discriminated strain BR102T from other closely related species of the genus Citrobacter . The novel variants bla CMY-179 and qnrB97, which encoded a CMY-2-like β-lactamase and a QnrB-type protein, respectively, were identified in strain BR102T. BR102T was resistant to ampicillin, amoxicillin/clavulanate and cefoxitin. The DNA G+C content of strain BR102T is 51.3 mol%. Based on these results, strain BR102T represents a novel species of the genus Citrobacter , for which the name Citrobacter meridianamericanus sp. nov. is proposed. The type strain is BR102T (=MUM 22.55T=IMI 507229T).
- Received:
- Accepted:
- Published Online:
Funding
-
Fundação para a Ciência e a Tecnologia
(Award UIDP/04378/2020, UIDB/04378/2020, and LA/P/0140/2020)
- Principle Award Recipient: PeixeLuísa
-
Fundação de Amparo à Pesquisa do Estado de São Paulo
(Award 2020/14426-3)
- Principle Award Recipient: HamiltonCabral
-
Fundação de Amparo à Pesquisa do Estado de São Paulo
(Award 2021/01655-7)
- Principle Award Recipient: ElianaGuedes Stehling