1887

Abstract

A Gram-stain-negative strain, designated BR102, isolated from a soil sample in Brazil was characterized by a polyphasic approach. Comparative 16S rRNA gene sequences indicated that strain BR102 belonged to the genus . The - and whole-genome-based phylogeny, and multilocus sequence analysis based on concatenated partial , , and sequences strongly supported a clade encompassing strain BR102 and a strain from public database that was distinct from currently recognized species of the genus . Average nucleotide identity and digital DNA–DNA hybridization values between strain BR102 and the closest relative ATCC 8090 were 91.8 and 48.8 %, respectively. The ability to metabolize different compounds further discriminated strain BR102 from other closely related species of the genus . The novel variants and , which encoded a CMY-2-like β-lactamase and a QnrB-type protein, respectively, were identified in strain BR102. BR102 was resistant to ampicillin, amoxicillin/clavulanate and cefoxitin. The DNA G+C content of strain BR102 is 51.3 mol%. Based on these results, strain BR102 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BR102 (=MUM 22.55=IMI 507229).

Keyword(s): Citrobacter , CMY-179 , Enterobacterales , qnrB and soil
Funding
This study was supported by the:
  • Fundação para a Ciência e a Tecnologia (Award UIDP/04378/2020, UIDB/04378/2020, and LA/P/0140/2020)
    • Principle Award Recipient: PeixeLuísa
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (Award 2020/14426-3)
    • Principle Award Recipient: HamiltonCabral
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (Award 2021/01655-7)
    • Principle Award Recipient: ElianaGuedes Stehling
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/content/journal/ijsem/10.1099/ijsem.0.005720
2023-03-21
2024-05-11
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