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Volume 73, Issue 2

sp. nov., a halotolerant bacterium isolated from woodland soil No Access

Abstract

A novel Gram-positive, aerobic, rod-shaped, non-motile, endospore-forming salt-tolerant bacterium strain (80), was isolated from woodland soil collected near Kanas lake in the Altay region of Xinjiang, PR China. The strain grew at 15−45 °C, pH6.0–9.0 and with 0–14 % (w/v) NaCl. The complete genome size of the novel strain was 4 031 766 bp including a circle chromosome and a circle plasmid. The genomic DNA G+C content was 38.99 mol %. Phylogenetic analysis based on 16S rRNA gene sequence and genome showed that strain 80 has the highest similarity to TKL69. However, the novel strain showed an average nucleotide identity value of 78.65 % (lower than 95 %) and a digital DNA–DNA hybridization value of 22.30 % with TKL69 based on the genome sequences. The major fatty acids were anteiso-C, iso-C, anteiso-C and C ω7 alcohol. The only respiratory quinone was MK-7. The cell wall peptidoglycan was -diaminopimelic acid. Diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid, one unidentified aminophospholipid and two unidentified glycolipids were identified as the major polar lipids. The phylogenetic, phenotypic and chemotaxonomic analyses showed that strain 80 represents a novel species of the genus and the name sp. nov. is proposed. The type strain is 80 (=GDMCC 1.2844=JCM 35077).

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  • Accepted:
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Keyword(s): halotolerant and Radiobacillus
Funding
This study was supported by the:
  • the Project of Renovation Capacity Building for the Young Sci-Tech Talents Sponsored by Xinjiang Academy of Agricultural Science (Award XJNKQ-2019019)
    • Principle Award Recipient: LijuanZhang
  • the Opening Project of Key Laboratory in Xinjiang (Award 2020D04032)
    • Principle Award Recipient: WeiWang
  • National Key Research and Development Program of China (Award 2020YFC1806603)
    • Principle Award Recipient: WeiWang
© 2023 The Authors
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2023-02-23
2024-05-07
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Radiobacillus kanasensis sp. nov., a halotolerant bacterium isolated from woodland soil
Int J Syst Evol Microbiol 73, 005718 (2023); https://doi.org/10.1099/ijsem.0.005718
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