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Volume 73, Issue 2

sp. nov., isolated from poultry farm soil No Access

Abstract

Strain SX5 was isolated from the soil of a poultry farm in Shanxi Province, PR China. The isolate was a Gram-stain-negative, rod-shaped, non-flagellated, and yellow bacterium. Growth occurred at 20–37 °C (optimum, 28 °C), pH 6.0–10.0 (optimum, pH 8.0) and 0–1 % NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SX5 was related to members of the genus , and close to H23 (97.9 %), Y4 (97.9 %), RIB1-20 (96.8 %), SYP-B804 (96.4 %) and Gsoil 068 (96.1 %). The major cellular fatty acids of strain SX5 were iso-C, iso-C 9, iso-C and iso-C 3OH. The sole isoprenoid quinone was ubiquinone Q-8, and the major polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Genome analyses revealed that strain SX5 had a genome size of 3.6 Mbp with a G+C content of 65.7 mol% and contained abundant carbohydrate-active enzyme genes and three putative distinct biosynthetic gene clusters, suggesting that it may have great potential to degrade and utilize complex biological organic matter and produce special secondary metabolites. Comparative genomic analyses clearly separated strain SX5 from the known species of the genus based on average nucleotide identity and digital DNA–DNA hybridization values below the thresholds for species delineation. Based on its phenotypic, genotypic properties and phylogenetic inference, strain SX5 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is SX5 (=GDMCC 1.2162=KCTC 82443=JCM 34401).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , genome analysis , Luteimonas galliterrae and polyphasic taxonomy
Funding
This study was supported by the:
  • University–Industry Collaborative Education Program (Award 202102083002)
    • Principle Award Recipient: Guo-zhuZhao
  • Fundamental Research Funds for the Central Universities at Beijing Forestry University (Award 2021ZY61)
    • Principle Award Recipient: Guo-zhuZhao
  • National Natural Science Foundation of China (Award 31570019)
    • Principle Award Recipient: Guo-zhuZhao
  • National Natural Science Foundation of China (Award 31770110)
    • Principle Award Recipient: Xiang-weiHe
© 2023 The Authors
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2024-05-19
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Luteimonas galliterrae sp. nov., isolated from poultry farm soil
Int J Syst Evol Microbiol 73, 005717 (2023); https://doi.org/10.1099/ijsem.0.005717
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