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Volume 73, Issue 2

sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from mangrove sediment No Access

Abstract

Polycyclic aromatic hydrocarbons (PAHs) have been recognized as a potential health risk and are widespread in nature due to their intrinsic chemical stability and high recalcitrance to degradation. A taxonomic study was carried out on strain P9, which was isolated from a PAH-degrading consortium, enriched from the mangrove sediment from Zhangzhou, PR China. The isolate was chemoheterotrophic, aerobic, Gram-stain-negative, short-rod shaped, and motile by one polar flagellum. Growth was observed at salinities from 0.5–6.0 % (optimum, 3 %), at pH 4–9 (optimum, pH 7) and at 10–41 °C (optimum, 25–30 °C). It did not synthesize bacteriochlorophyll a. Catalase and oxidase activities were positive. Acid was produced from starch, amygdalin, arbutin, cellobiose, -fructose, maltose, -mannitol, melezitose, melibiose, raffinose, -ribose, sucrose, trehalose, -xylose, aesculin ferric citrate, gentiobiose, glycogen, -arabinose, -rhamnose, methyl --glucopyranoside, methyl --xylopyranoside, -acetylglucosamine and salicin, and weakly positive for -arabitol, -galactose, lactose, turanose and glycerol. Phylogenetic analysis revealed that strain P9 fell within the clade comprising the type strains of species and formed an independent cluster with , which was distinct from other members of the family . The 16S rRNA gene sequence comparisons showed that strain P9 was most closely related to HTCC 260 (96.7 %), and other species of the genus (95.7–94.2 %). Strain P9 had the highest digital DNA–DNA hybridization value with CGMCC 1.12377 (25.0 %) and the highest average nucleotide identity (ANIb and ANIm) values with CGMCC 1.12377(80.3 and 85.8 %, respectively). The sole respiratory quinone was quinone 10. The dominant fatty acids were C 7 (61.4 %), C (17.5 %) and C 8 cyclo (7.6 %). The G+C content of the chromosomal DNA was 65.8 mol%. In the polar lipid profile, phospholipid, phosphatidylglycerol, aminolipid, glycolipid and phosphatidylethanolamine were the major compounds. Based on the phenotypic and phylogenetic data, strain P9 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is P9 (=CCTCC AB 209290=LMG 25701=MCCC 1F01055).

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  • Accepted:
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Keyword(s): mangrove sediment , novel species , polycyclic aromatic hydrocarbon and Salipiger
Funding
This study was supported by the:
  • FJIRSM and IUE Joint Research Fund (Award RHZX-2019-005)
    • Principle Award Recipient: Chang-PingYu
  • National Natural Science Foundation of China (Award 31870475)
    • Principle Award Recipient: AnyiHu
  • Fujian Province priority project (Award 2020Y0086)
    • Principle Award Recipient: DanQin
  • STS Project of Fujian-CAS (Award 2021T3059)
    • Principle Award Recipient: DanQin
  • STS Project of Fujian-CAS (Award 2021T3014)
    • Principle Award Recipient: JiangweiLi
© 2023 The Authors
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2023-02-15
2024-05-19
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Salipiger pentaromativorans sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from mangrove sediment
Int J Syst Evol Microbiol 73, 005716 (2023); https://doi.org/10.1099/ijsem.0.005716
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