1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 3

sp. nov. and sp. nov., two novel species isolated from , show genomic and physiological adaptations for a -associated lifestyle No Access

Abstract

The genus from the currently includes six validated species. Two strains designated PT2-4 and 62-3 were isolated from abundant at the Pingtan island coast in the Fujian Province of China. 16S rRNA gene sequence analysis showed that the closest described relative of strains PT2-4 and 62-3 is JCM 19808 with 98.40 and 97.98% sequence similarity, respectively. The 16S rRNA gene sequence similarity between strain PT2-4 and strain 62-3 was 98.68 %. Furthermore, the highest average nucleotide identity values were 87.34 and 88.97 % for strains PT2-4 and 62-3, respectively. The highest DNA–DNA hybridization (DDH) value of strain PT2-4 was 35.2 % with strain 62-3, while the DDH value of strain 62-3 was 37.7 % with JCM 19808. Growth of strains PT2-4 and 62-3 occurs at 15–40 °C (optimum, 30 °C) with 0–4 % (w/v) NaCl (optimum 0–1 %). Strains PT2-4 and 62-3 can grow from pH 5.0 to 10.0 (optimum, pH 7.0). The major fatty acids of strains PT2-4 and 62-3 are iso-C and iso G-C. MK-6 is the sole respiratory quinone. Genomic and physiological analyses of strains PT2-4 and 62-3 showed corresponding adaptive features. Significant adaptation to the growth environment of macroalgae includes the degradation of brown algae-derived diverse polysaccharides (alginate, laminarin and fucoidan). Notably, strain PT2-4 can utilize laminarin, fucoidan and alginate via specific carbohydrate-active enzymes encoded in polysaccharide utilization loci, rarely described for the genus to date. Based on their distinct physiological characteristics and the traits of utilizing polysaccharides from , strains PT2-4 and 62-3 are suggested to be classified into two novel species, sp. nov. and sp. nov. (type strain PT2-4=MCCC 1K04427=KCTC 92183 and type strain 62-3=MCCC 1K04421=KCTC 92182).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CAZymes , novel species , PULs , Sargassum association and Tamlana
Funding
This study was supported by the:
  • Major Project of Talent Team Introduction for Guangdong Provincial Laboratory of Southern Marine Science and Engineering (Guangzhou) (Award GML2019ZD0606)
    • Principle Award Recipient: ZhongHu
  • Innovation Team Project of Guangdong Normal University (Award 2018KCXTD012)
    • Principle Award Recipient: ZhongHu
  • Science and Technology Planning Project of Shantou City (Award 2020ST011)
    • Principle Award Recipient: ZhongHu
  • Basic and Applied Basic Research Foundation of Guangdong Province (Award 2021A1515111097)
    • Principle Award Recipient: JinLi
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005706
2023-03-08
2024-04-28
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Tamlana laminarinivorans sp. nov. and Tamlana sargassicola sp. nov., two novel species isolated from Sargassum, show genomic and physiological adaptations for a Sargassum-associated lifestyle
Int J Syst Evol Microbiol 73, 005706 (2023); https://doi.org/10.1099/ijsem.0.005706
/content/journal/ijsem/10.1099/ijsem.0.005706
/content/journal/ijsem/10.1099/ijsem.0.005706
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