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Volume 73, Issue 1

gen. nov., sp. nov., a plant polysaccharide-decomposing anaerobic bacterium isolated from anoxic soil subjected to reductive soil disinfestation, and reclassification of as comb. nov. No Access

Abstract

An obligately anaerobic bacterial strain (TB5) was isolated from a soil sample subjected to reductive or biological soil disinfestation. Cells of the strain were Gram-stain-positive, spore-forming and motile rods. The strain grew at 15–40 °C (optimum, 37 °C) and pH 5.4–7.5 (optimum, pH 7.3). Strain TB5utilized a wide variety of carbohydrates including polysaccharides (cellulose, xylan, starch, inulin, glucomannan and laminarin) and organic acids. Acetate, ethanol, H and CO were products from the substrates utilized. The major components of the cellular fatty acids were C 7 DMA, C DMA and C 7 DMA. The diagnostic amino acid of the cell-wall peptidoglycan was -diaminopimelic acid. The closest related species to strain TB5 based on 16S rRNA gene sequences was 743A (95.4 % sequence similarity). The genome size of strain TB5 was 5.09 Mb and the genomic DNA G+C content was 32.7 mol%. Strain TB5 had genes encoding polysaccharide-decomposing enzymes such as cellulase, xylanase, β-glucosidase and β-mannosidase in the genome. Based on the phylogenetic, genomic and phenotypic data, a novel species of a novel genus in the family , gen. nov., sp. nov., is proposed to accommodate the strain. The type species is with strain TB5 (=NBRC 112093=DSM 110037) as the type strain. For the closest related species , comb. nov. is proposed with an emended description of the species.

  • Received:
  • Accepted:
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Keyword(s): Anaeromicropila herbilytica , biological soil disinfestation , Clostridium populeti , plant polysaccharide decomposition and reductive soil disinfestation
Funding
This study was supported by the:
  • Ministry of Agriculture, Forestry and Fisheries of Japan (Science and technology research promotion program for agriculture, forestry, fisheries and food industry) (Award 27016C)
    • Principle Award Recipient: UekiAtsuko
© 2023 The Authors
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2023-01-27
2024-04-26
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Anaeromicropila herbilytica gen. nov., sp. nov., a plant polysaccharide-decomposing anaerobic bacterium isolated from anoxic soil subjected to reductive soil disinfestation, and reclassification of Clostridium populeti as Anaeromicropila populeti comb. nov.
Int J Syst Evol Microbiol 73, 005695 (2023); https://doi.org/10.1099/ijsem.0.005695
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