1887

Abstract

A Gram-stain-negative, strictly aerobic, motile, rod-shaped bacterium, FT117, was isolated from mangrove sediment collected in Shenzhen, Guangdong, PR China. Growth occurred at 20–45 °C (optimum, 40 °C), pH 6–10 (optimum, 8) and in the presence of 0–5 % NaCl (optimum, 0 %). The results of 16S rRNA gene sequence analysis indicated that the identity between FT117 and CC-CFT501 was the highest (98.7 %), followed by JCM 31785 (93.6 %), CCUG 34794 (93.6 %) and LMG 31012 (93.5 %). The main fatty acids (>10 %) were C (35.8 %), cyclo-C (18.5 %) and summed feature 3 (18.1 %). The polar lipids contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentified aminophospholipids and one unidentified lipid. The sole respiratory quinone was ubiquinone-8 (Q-8). Its DNA G+C content was 70.6 % (data from the genome sequence) and the estimated genome size was 3.860 Mb. The average nucleotide identity values between the FT117 genome and the genomes of CC-CFT501, JCM 31785, CCUG 34794 and LMG 31012 were 85.4 %, 76.4 %, 73.0 % and 71.3 %, and the digital DNA–DNA hybridization values were 29.1 %, 21.0 %, 20.3 % and 19.1 %, respectively. The phylogenetic, phenotypic and chemotaxonomic differences between FT117 and its phylogenetic relatives indicate that FT117 should be regarded as representing a novel species within the genus , for which the name sp. nov. is proposed. The type strain is FT117 (=KCTC 92314 = MCCC 1K07396).

Funding
This study was supported by the:
  • Shenzhen Science and Technology Program (Award Grant no. JCYJ20200109105010363)
    • Principle Award Recipient: MengLi
  • Innovation Team Project of Universities in Guangdong Province (Award No. 2020KCXTD023)
    • Principle Award Recipient: MengLi
  • National Science and Technology Fundamental Resources Investigation Program of China (Award Grant No. 2019FY100700)
    • Principle Award Recipient: MengLi
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2023-01-27
2024-11-10
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