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Volume 73, Issue 1

sp. nov., isolated from bovine faeces in Northern Ireland Open Access

Abstract

A new species of , a Gram-positive, spore-forming anaerobic group, proposed name sp. nov., was isolated in Northern Ireland from bovine faeces collected in 2016. Designated as MCA3, cells of sp. nov. are rod shaped and motile. Cells tolerate NaCl from 0.5 to 5.5 % (w/v), with a pH tolerance between pH 6 and 9. The optimal temperature for growth is 35–40 °C, and temperatures from 20 to 30 °C are tolerated. The polar lipid profile displays diphosphatidylglycerol, phosphatidylglycerol, two aminoglycolipids, one glycophospholipid, one aminolipid, three glycolipids, five phospholipids and one lipid. No respiratory quinones are detected. The predominant fatty acid profile includes C at 22.8 %. Strain MCA3 is positive for glucose and maltose acidification, as well as glycerol and sorbitol. The biochemical results from a VITEK2 assay of strain MCA3, LAM0A37 and DSM 6539 are also included for the first time. The closed and complete genome of strain MCA3 from a hybrid Oxford Nanopore Technology MinION/Illumina assembly reveals no evidence for known virulence genes. Draft genome sequencing of DSM 6539 and LAM0A37, as performed by Illumina MiSeq, provides reference genomes for these respective species of for the first time. DNA–DNA hybridization values (d) of MCA3 to ATCC 14880, LAM0A37 and DSM 6539 are 48.8, 67.4 and 46.3 %, with cutoff value at 70 %. The type strain for sp. nov. is MCA3 (=NCTC 14625=LMG 32430).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Illumina MiSeq , ONT MinION , phylogeny , Terrisporobacter , Terrisporobacter hibernicus and WGS
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-01-10
2024-04-28
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Terrisporobacter hibernicus sp. nov., isolated from bovine faeces in Northern Ireland
Int J Syst Evol Microbiol 73, 005667 (2023); https://doi.org/10.1099/ijsem.0.005667
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