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Volume 73, Issue 1

sp. nov. and sp. nov., isolated from a marine red alga and emended description of the genus No Access

Abstract

Two Gram-stain-negative, strictly aerobic, catalase- and oxidase-positive and non-motile rod-shaped bacteria, strains D2-3 and G9-8, were isolated from a marine red alga. Both strains contained ubiquinone-10 as the sole isoprenoid quinone. As the major cellular fatty acids (>5.0 %), D2-3 contained C, 11-methyl-Cω7, summed feature 3 (Cω7 and/or Cω6), and summed feature 8 (Cω7 and/or Cω6), whereas G9-8 contained C, 11-methyl-Cω7, C 3-OH, and summed feature 8. The DNA G+C contents of D2-3 and G9-8 were 54.4 % and 56.0 %, respectively. As the major polar lipids, phosphatidylglycerol, diphosphatidylglycerol and unidentified phospholipid, aminolipid and lipid were identified from both strains, and phosphatidylcholine was additionally detected from G9-8 only. The 16S rRNA gene sequence similarity of D2-3 and G9-8 was 98.5 % and their digital DNA–DNA hybridization (DDH) value was 19.1 %. Phylogenetic analyses based on 16S rRNA gene and genome sequences revealed that D2-3 and G9-8 formed respectively distinct phylogenetic lineages within the genus . D2-3 and G9-8 were most closely related to RA1-3 and 307, with 98.9 % and 98.5 % 16S rRNA gene sequence similarities, respectively, and digital DDH values between D2-3 and and between G9-8 and were 18.3 % and 19.5 %, respectively. Phenotypic, chemotaxonomic and molecular features support the hypothesis that D2-3 and G9-8 represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains of and are D2-3 (=KACC 22493 =JCM 34969) and G9-8 (=KACC 22488 =JCM 34973), respectively.

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  • Accepted:
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Keyword(s): marine red alga , novel taxa , Octadecabacter algicola , Octadecabacter dasysiphoniae and Pseudomonadota
Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR202129201)
    • Principle Award Recipient: CheOk Jeon
  • Korea Institute of Ocean Science and Technology (Award KIOST20220469)
    • Principle Award Recipient: CheOk Jeon
© 2023 The Authors
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2024-05-14
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References

  1. Gosink JJ, Herwig RP, Staley JT. Octadecabacter arcticus gen. nov., sp. nov., and O. antarcticus, sp. nov., nonpigmented, psychrophilic gas vacuolate bacteria from polar sea ice and water. Syst Appl Microbiol 1997; 20:356–365 [View Article]
     [Google Scholar]
  2. Billerbeck S, Orchard J, Tindall BJ, Giebel H-A, Brinkhoff T et al. Description of Octadecabacter temperatus sp. nov., isolated from the southern North Sea, emended descriptions of the genus Octadecabacter and its species and reclassification of Octadecabacter jejudonensis Park and Yoon 2014 as Pseudooctadecabacter jejudonensis gen. nov., comb. nov. Int J Syst Evol Microbiol 2015; 65:1967–1974 [View Article]
     [Google Scholar]
  3. Kim Y-O, Park I-S, Park S, Nam B-H, Park J-M et al. Octadecabacter ascidiaceicola sp. nov., isolated from a sea squirt (Halocynthia roretzi). Int J Syst Evol Microbiol 2016; 66:296–301 [View Article] [PubMed]
     [Google Scholar]
  4. Park S, Yoon SY, Jung YT, Yoon JH. Octadecabacter ponticola sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2016; 66:4179–4184 [View Article]
     [Google Scholar]
  5. Kim KH, Kristyanto S, Kim HM, Kim KR, Jeon CO. Nitratireductor rhodophyticola sp. nov., isolated from marine red algae. Int J Syst Evol Microbiol 2022; 72:005302 [View Article] [PubMed]
     [Google Scholar]
  6. Khan SA, Jeong SE, Baek JH, Jeon CO. Maribacter algicola sp. nov., isolated from a marine red alga, Porphyridium marinum, and transfer of Maripseudobacter aurantiacus Chen et al. 2017 to the genus Maribacter as Maribacter aurantiacus comb. nov. Int J Syst Evol Microbiol 2020; 70:797–804 [View Article]
     [Google Scholar]
  7. Kim J, Kim KH, Chun BH, Khan SA, Jeon CO. Flagellimonas algicola sp. nov., isolated from a marine red alga, Asparagopsis taxiformis. Curr Microbiol 2020; 77:294–299 [View Article]
     [Google Scholar]
  8. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article] [PubMed]
     [Google Scholar]
  9. Nawrocki EP, Eddy SR. Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 2007; 3:e56 [View Article]
     [Google Scholar]
  10. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
     [Google Scholar]
  11. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. Bioinformatics 2014; 30:2114–2120
     [Google Scholar]
  12. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article] [PubMed]
     [Google Scholar]
  13. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP et al. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 2016; 44:6614–6624 [View Article] [PubMed]
     [Google Scholar]
  14. Zhang H, Yohe T, Huang L, Entwistle S, Wu P. DbCAN2: a meta server for automated carbohydrate-active enzyme annotation. Nucleic Acids Res 2012; 40:W445–W451
     [Google Scholar]
  15. Dogs M, Wemheuer B, Wolter L, Bergen N, Daniel R et al. Rhodobacteraceae on the marine brown alga Fucus spiralis are abundant and show physiological adaptation to an epiphytic lifestyle. Syst Appl Microbiol 2017; 40:370–382 [View Article] [PubMed]
     [Google Scholar]
  16. Tashiro Y, Monson RE, Ramsay JP, Salmond GPC. Molecular genetic and physical analysis of gas vesicles in buoyant enterobacteria. Environ Microbiol 2016; 18:1264–1276 [View Article] [PubMed]
     [Google Scholar]
  17. Kim J, Na S-I, Kim D, Chun J. UBCG2: Up-to-date Bacterial Core Genes and pipeline for phylogenomic analysis. J Microbiol 2021; 59:609–615 [View Article] [PubMed]
     [Google Scholar]
  18. Lee I, Ouk Kim Y, Park S-C, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article] [PubMed]
     [Google Scholar]
  19. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
     [Google Scholar]
  20. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci 2009; 106:19126–19131 [View Article]
     [Google Scholar]
  21. Gomori G. Preparation of buffers for use in enzyme studies. Methods Enzymol 1955; 1:138–146
     [Google Scholar]
  22. Bernardet J-F, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article]
     [Google Scholar]
  23. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. eds Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
     [Google Scholar]
  24. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19:1–67
     [Google Scholar]
  25. Komagata K, Suzuki KI. Lipid and cell- wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–208
     [Google Scholar]
  26. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty acids, MIDI Technical note 101 Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  27. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117 [View Article]
     [Google Scholar]
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Octadecabacter algicola sp. nov. and Octadecabacter dasysiphoniae sp. nov., isolated from a marine red alga and emended description of the genus Octadecabacter
Int J Syst Evol Microbiol 73, 005664 (2023); https://doi.org/10.1099/ijsem.0.005664
/content/journal/ijsem/10.1099/ijsem.0.005664
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