1887

Abstract

Two novel strains, designated XJ19-45 and XJ19-1, were isolated from water of Kuche River in Xinjiang Uygur Autonomous Region, China. Their cells were Gram-stain-negative, aerobic and motile rods. The phylogenetic analyses based on 16S rRNA genes and genomes showed that the two isolates belonged to the genus and the closest relative was HST3-14. The 16S rRNA genes sequences pairwise similarities, average nucleotide identities, digital DNA–DNA hybridizations and average amino acid identities between type strain XJ19-45 and other relatives were all less than 98.3, 80.3, 23.6 and 85.7 %, respectively, all below the species delineation thresholds. Pan-genomic analysis indicated that the novel isolate XJ19-45 shared 1594 core gene clusters with the 11 closely related type strains in , and the number of strain-specific clusters was 390. The major cellular fatty acids (>10 %) of the two isolates were summed feature 8, C 7c 11-methyl and C. Diphosphatidylglycerol, phosphatidylglycerol and glycolipids were the major polar lipids, and Q10 was the detected respiratory quinone. Based on the results of phenotypic, physiological, chemotaxonomic and genotypic characterizations, we propose that the isolates represent a novel species, for which the name sp. nov. is proposed. The type strain is XJ19-45 (=CGMCC 1.19388=KCTC 92263).

Funding
This study was supported by the:
  • National Key Research Program of China (Award 2019YFE0191000)
    • Principle Award Recipient: ManCai
  • National Nature Science Foundation of China (Award 32170101)
    • Principle Award Recipient: ManCai
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: Bao-ZhuFang
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2022-12-20
2024-05-30
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