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Volume 72, Issue 12

sp. nov., isolated from Kubuqi Desert soil No Access

Abstract

A Gram-stain positive, facultatively anaerobic, motile rod-shaped strain, BY-33, was isolated from a soil sample obtained from the Kubuqi Desert, PR China. Phylogenetic analysis based on 16S rRNA gene sequence similarity revealed that strain BY-33 was most closely related to the genus , including CF5-4 (98.2 % similarity), ‘’ HO-Ch2 (98.0 %), THG-T121 (97.6 %), ‘’ 69B4 (97.5 %), M (97.3 %) and ‘’ T26 (97.0 %). The strain grew at 0‒37 °C (optimum, 28–30 °C) and pH 6.0–11.0 (optimum, pH 9.0–10.0) and with 0‒8.0 % (w/v) NaCl (optimum, 3.0%) on tryptic soy agar. It had catalase activity, but no oxidase activity. The polar lipids of strain BY-33 contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and phosphatidylinositol mannosides. The major respiratory quinone of strain BY-33 was MK-10 (H). Its major fatty acids were anteiso-C, anteiso-C A and C. The genomic DNA G+C content of strain BY-33 was 73.0 mol% based on total genome calculations. The average nucleotide identity scores between the genomic sequences of strain BY-33 and the other species of the genus were found to be low (ANIm <85.0 %, ANIb <77.0 % and OrthoANIu <78.0 %). Furthermore, the digital DNA–DNA hybridization and average amino acid identity values between strain BY-33 and the closely related species ranged from 20.5 to 21.0% and from 62.2 to 72.2 %, respectively. Based on the results of phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain BY-33 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BY-33 (=CGMCC 1.17460=KCTC 49362).

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Keyword(s): 16S rRNA gene , Actinotalea soli sp. nov. and polyphasic taxonomy
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31070002)
    • Principle Award Recipient: ZhangJianli
  • Beijing Natural Science Foundation (Award 5152017)
    • Principle Award Recipient: CuiSiqi
  • National Natural Science Foundation of China (Award 31070002)
    • Principle Award Recipient: BaiPengze
  • National Natural Science Foundation of China (Award 32172126)
    • Principle Award Recipient: PangHuancheng
  • Beijing Natural Science Foundation (Award 5152017)
    • Principle Award Recipient: ChenYa
  • National Natural Science Foundation of China (Award 31070002)
    • Principle Award Recipient: PingWeiwei
  • National Natural Science Foundation of China (Award 31070002)
    • Principle Award Recipient: DuJie
© 2022 The Authors
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2022-12-20
2024-05-09
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Actinotalea soli sp. nov., isolated from Kubuqi Desert soil
Int J Syst Evol Microbiol 72, 005660 (2023); https://doi.org/10.1099/ijsem.0.005660
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