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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 11

sp. nov., a novel microcystin-degrading alphaproteobacterium containing the carbapenemase gene No Access

Abstract

A Gram-stain-negative, rod-shaped, microcystin-degrading bacterium, designated as CPCC 100929, was isolated from a fresh water reservoir in Sichuan Province, PR China. This isolate grew well at 4–37 °C and pH 6.0–8.0, with optimal growth at 28–32 °C and pH 7.0, respectively. The major cellular fatty acids were C ω7/C ω6, C, C ω7 11-methyl and C cyclo ω8. The predominant respiratory quinone was Q-10. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine and phosphatidylcholine were detected in the polar lipids extraction. The 16S rRNA gene sequence of strain CPCC 100929 was closely related to those of members of the genus , with the highest similarity of 98.6 % to DSM 287 and 97.4–98.4 % with other identified members. In the phylogenetic trees based on 16S rRNA gene sequences and the core-genes analysis, strain CPCC 100929 was included within the clade of the genus . The values of average nucleotide identity (81.4–86.7 %) and digital DNA–DNA hybridization (25.4–44.6 %) between strain CPCC 100929 and other species were all below the thresholds for bacterial species delineation, respectively. The genomic DNA G+C content of strain CPCC 100929 was 63.6 %. The genomic sequence analysis indicated that this species contained genes encoding peroxidase, carbapenemase and the key enzyme for microcystin bio degradation, as well as rich carbohydrate-active enzyme coding genes, which might endow the micro-organism with properties to adapt to diverse environments. Based on its phenotypic and genetic properties, we propose that strain CPCC 100929 (=T1A350=KCTC 72957) is the type strain of a novel species with the name sp. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , genome , polyphasic taxonomy and Shinella lacus
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32170021)
    • Principle Award Recipient: ZhangYuqin
© 2022 The Authors
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2022-11-10
2024-05-18
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Shinella lacus sp. nov., a novel microcystin-degrading alphaproteobacterium containing the bla carbapenemase gene
Int J Syst Evol Microbiol 72, 005659 (2022); https://doi.org/10.1099/ijsem.0.005659
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