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Volume 73, Issue 1

sp. nov., a nitrogen-fixing actinobacterium isolated from Open Access

Abstract

A nitrogen-fixing actinobacterium strain (Cc1.17) isolated from a root nodule of was subjected to polyphasic taxonomic studies. The strain was characterized by the presence of -diaminopimelic acid in its peptidoglycan, galactose, glucose, mannose, rhamnose, ribose and xylose as cell-wall sugars, phosphatidylinositol, diphosphatidylglycerol, glycophospholipids, phosphatidylglycerol, glycophospholipid and uncharacterized lipids as its polar lipids, and C, isoC, C ω9 and C 9 as major fatty acids (>10 %). Strain Cc1.17 showed 16S rRNA gene sequence similarities of 97.4–99.8 % to validly named species. Phylogenetic trees based on 16S rRNA gene and genome sequences placed strain Cc1.17 in a new lineage within the genus . Digital DNA–DNA hybridization and average nucleotide identity values between strain Cc1.17 and its closest phylogenomic neighbours were well below the thresholds recommended for prokaryotic species delineation. Therefore, strain Cc1.17 (=DSM 43829=CECT 9313) merits recognition as the type strain of a new species for which the name sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Frankia , phylogeny , polyphasic taxonomy and symbiosis
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-01-27
2024-04-29
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Frankia colletiae sp. nov., a nitrogen-fixing actinobacterium isolated from Colletia cruciata
Int J Syst Evol Microbiol 73, 005656 (2023); https://doi.org/10.1099/ijsem.0.005656
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