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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 72, Issue 12

sp. nov., isolated from saline-alkali soil No Access

Abstract

A Gram-stain-negative, aerobic and rod-shaped bacterium, strain Y2R2, was isolated from a saline–alkali soil sample collected from Binhai New Area, Tianjin, PR China. Growth of strain Y2R2 was observed at 10–45 °C (optimum, 30 °C), at pH 6.0–11.0 (optimum, pH 9.0) and in the presence of 0–15 % (w/v) NaCl (optimum, 9.0 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Y2R2 was affiliated with the genus and showed the highest similarity to BJGMM-B45 (99.0%) and DSM 4740 (98.4%). The digital DNA–DNA hybridization and average nucleotide identity values of 21.0–22.8 % and 73.3–75.7 % with the closely related species BJGMM-B45, DSM 4740, AL12, N12 and SYSU ZJ2214 were lower than the threshold recommended for species discrimination.

The major respiratory quinone of strain Y2R2 was Q-9 and the major cellular fatty acids consisted of C, C cyclo ω8 and summed feature 8 (C ω7 and/or C ω6). The DNA G+C content of strain Y2R2 was 57.0 mol%. On the basis of this polyphasic taxonomic study, strain Y2R2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Y2R2 (=CGMCC 1.16974=KCTC 72578).

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  • Accepted:
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Keyword(s): Halomonas binhaiensis , Proteobacteria and taxonomy
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31571790)
    • Principle Award Recipient: XiaoyanLi
  • Tianjin Agricultural University Graduate Scientific Research and Innovation Project (Award 2021XY029)
    • Principle Award Recipient: HegangLu
© 2022 The Authors
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2022-12-20
2025-01-18
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Halomonas binhaiensis sp. nov., isolated from saline-alkali soil
Int J Syst Evol Microbiol 72, 005652 (2023); https://doi.org/10.1099/ijsem.0.005652
/content/journal/ijsem/10.1099/ijsem.0.005652
/content/journal/ijsem/10.1099/ijsem.0.005652
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