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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 12

sp. nov., isolated from midden soil waste No Access

Abstract

A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area. According to the results of a polyphasic taxonomic study, a novel species belonging to the genus was described. Strain ABSL32-1 grew optimally at 20–25 °C and at pH 6.0–8.0 in the presence of 1 % (w/v) NaCl. The whole-cell sugars were ribose, mannose and glucose. The peptidoglycan structure contained A4α peptidoglycan (Lys–Glu; A11.54). The polar lipids contained digalactosyldiacylglycerol, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and two unidentified lipids. The major menaquinones were MK-9 and MK-10. The major cellular fatty acid was anteiso-C (70.1 %). Based on 16S rRNA gene sequence analysis, strain ABSL32-1 showed the highest similarity to DSM 20167 (99.5 %), followed by SPC26 (99.0 %) and AG31 (98.8 %). The genome of strain ABSL32-1 is 4.4 Mbp with a DNA G+C content of 66.0 mol%. The average nucleotide identity values between strain ABSL32-1 and the type strains DSM20167, SPC26 and AG31 were 86.6, 74.7 and 83.6 %, respectively. On the basis of phenotypic, chemotaxonomic and genotypic properties, strain ABSL32-1 is proposed to represent a novel species to be named sp. nov. The type strain is ABSL32-1 (=TBRC 14976=NBRC 115252).

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Keyword(s): actinomycete , midden soil waste and Paeniglutamicibacter quisquiliarum
Funding
This study was supported by the:
  • the Ratchadapisek Somphot Fund for Postdoctoral Fellowships, Chulalongkorn University
    • Principle Award Recipient: OnruthaiPinyakong
© 2022 The Authors
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2024-05-09
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Paeniglutamicibacter quisquiliarum sp. nov., isolated from midden soil waste
Int J Syst Evol Microbiol 72, 005651 (2023); https://doi.org/10.1099/ijsem.0.005651
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