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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 12

sp. nov., a novel actinobacterium isolated from Antarctic tundra soil No Access

Abstract

A Gram-negative, non-motile, aerobic bacterium, named 02-257, was isolated from Antarctic soil. The cells are surrounded by relatively thin capsules and were catalase-positive and oxidase-negative cocci. Growth of strain 02-257 was observed at 4–35 °C (optimum, 28–30 °C), pH 6.0–8.0 (optimum, pH 6.0) and with 0–1.5% NaCl (optimum, 0 %). Strain 02–257 showed the highest 16S rRNA gene sequence similarity to Seoho-28 (95.06 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 02-257 is a member of a novel species belonging to the clade formed by members of the genus in the family . The DNA G+C content was 72.9 mol%. Strain 02-257 contained C-iso (23.0 %), C 9 (13.8 %), C (12.5 %) and C 9-iso (10.8 %) as major cellular fatty acids and menaquinone MK-7(H) was detected as the only isoprenoid quinone. Diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositole mannoside, phosphatidylinositole dimannoside, unidentified phosphoglycolipid, unidentified aminophospholipid, two unidentified phospholipids, three unidentified aminolipids and six unidentified lipids were the major polar lipids. -Diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain 02-257 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 02-257 (=CCTCC AB 2021030=KCTC 49619).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Antarctica , novel species , Paraconexibacter antarcticus and phylogenetic analysis
Funding
This study was supported by the:
  • Chinese Polar Scientific Strategy Research Fund (Award IC201706)
    • Principle Award Recipient: FangPeng
  • National Natural Science Foundation of China (Award 42076230)
    • Principle Award Recipient: FangPeng
  • R&D Infrastructure and Facility Development Program of the Ministry of Science and Technology of the People’s Republic of China (Award NIMR-2022-8)
    • Principle Award Recipient: FangPeng
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: FangPeng
© 2022 The Authors
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Paraconexibacter antarcticus sp. nov., a novel actinobacterium isolated from Antarctic tundra soil
Int J Syst Evol Microbiol 72, 005647 (2023); https://doi.org/10.1099/ijsem.0.005647
/content/journal/ijsem/10.1099/ijsem.0.005647
/content/journal/ijsem/10.1099/ijsem.0.005647
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