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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 2

sp. nov., an endophytic bacterium isolated from the root of No Access

Abstract

A novel endophytic bacterium, designated strain BGMRC 0089, was isolated from a surface-sterilized root of . Cells were observed to be Gram-negative, rod-shaped and motile with polar flagella. Strain BGMRC 0089 was found to grow optimally at 28–30 °C, pH 7.0–8.0 and in the presence of 1 % (w/v) NaCl. Strain BGMRC 0089 contained ubiquinone Q-10 and the predominant fatty acid was summed feature 8. The polar lipid profile of strain BGMRC 0089 was found to contain diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine and phosphatidylethanolamine. Based on the results of 16S rRNA gene analysis, this isolate has the closest phylogenetic relationships with L6-16 (96.5 %) and N19 (96.4 %). Average nucleotide identity, amino acid identity and digital DNA–DNA hybridization values of the isolate with the type strains of the genera and were below 84.6, 73.9 and 22.1  %, respectively. Analysis the 4.55 Mb draft genome of strain BGMRC 0089 revealed several plant-associated genes, which may play important roles for the plant in the adaptation to the mangrove habitat. Based on its distinct phylogenetic, phenotypic and chemotaxonomic characteristics, strain BGMRC 0089 is proposed to represent a novel species, for which the name sp. nov. is proposed (type strain BGMRC 0089=DSM 100171=MCCC 1K04805).

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  • Accepted:
  • Published Online:
Keyword(s): Allorhizobium , endophytic bacterium and taxonomy
Funding
This study was supported by the:
  • Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry (Award 17-259-74)
    • Principle Award Recipient: Shu-ShiHuang
  • This research was supported by the National Natural Science Foundation of China (Award 42066002)
    • Principle Award Recipient: Xian-LingQin
© 2023 The Authors
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2023-02-14
2024-05-07
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Allorhizobium sonneratiae sp. nov., an endophytic bacterium isolated from the root of Sonneratia apetala
Int J Syst Evol Microbiol 73, 005641 (2023); https://doi.org/10.1099/ijsem.0.005641
/content/journal/ijsem/10.1099/ijsem.0.005641
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