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Abstract

A filamentous cell-shaped halophilic archaeon (strain AD-1) was isolated from Aiding Salt Lake, PR China. Its colonies on HCM7 agar plates were pinkish white, 1–4 mm (diameter), elevated and round. The optimum conditions for growth were observed at 42 °C, 4.3 M NaCl, 0.01 M MgCl and pH 7. Strain AD-1 could hydrolyse Tween 60, Tween 80, starch and gelatin. Phylogenetic analysis based on 16S rRNA gene, rpoB′ and the concatenated 484 single-copy orthologous proteins revealed that strain AD-1 formed a clade with SPP-AMP-1. The average nucleotide identity and DNA–DNA hybridization values between strain AD-1 and SPP-AMP-1 were both below the species delineation thresholds (95~96 and 70 %, respectively). The major phospholipids of strain AD-1 were phosphatidic acid, phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, while the major glycolipids were sulphated galactosyl mannosyl glucosyl diether, galactosyl mannosyl glucosyl diether and glucosyl mannosyl glucosyl diether. The phenotypic, phylogenetic and genome-based analyses suggested that strain AD-1 (=CGMCC 1.13724=JCM 32960) represents a novel species, for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32070003)
    • Principle Award Recipient: Heng-LinCui
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/content/journal/ijsem/10.1099/ijsem.0.005637
2022-12-16
2024-07-25
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