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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 12

sp. nov. and sp. nov. isolated from No Access

Abstract

Four Gram-stain-positive, facultatively anaerobic, non-motile, non-spore-forming and rod-shaped bacteria (lx-72, lx-45, ZJ784 and ZJ955) were isolated from the respiratory tract or faeces of marmot () from the Qinghai–Tibet Plateau in China. Analysis of the 16S rRNA gene sequences showed that all strains belong to the genus and are more related to CCUG 64069 (95.1–97.4 % similarity) than to the genus . Both strain pairs grew well at pH 6–9 and 15–42°C, and ZJ784/ZJ955 could tolerate slightly higher NaCl (0.5–4.5 %, w/v) than lx-72/lx-45(0.5–3.5 %). Based on whole-genome sequences, the average nucleotide identity and digital DNA–DNA hybridization values between our four isolates and their closest relative were below the species delineation thresholds of 70 % and 95–96 %. The common major fatty acids (>10 %) of our four strains were anteiso-C and anteiso-C. For both new type strains, MK-8(H) and MK-9(H) were the major isoprenoid quinones, and diphosphatidylglycerol and phosphatidylglycerol were the main polar lipids. The genomic DNA G+C content of all strains was 53.9 mol%. Based on results from the genomic comparison, phylogenetic analysis, and physiological and biochemical characteristics, the four isolates represent two novel species in the genus , for which the names sp. nov. (type strain lx-72=KCTC 49658=GDMCC 1.2569) and sp. nov. (type strain ZJ784=KCTC 49507=GDMCC 1.1997) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Canibacter , Marmota himalayana , novel species and Qinghai–Tibet Plateau
Funding
This study was supported by the:
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: JianguoXu
  • National Key R&D Program of China (Award 2019YFC1200505)
    • Principle Award Recipient: JingYang
  • National Key R&D Program of China (Award 2019YFC1200500)
    • Principle Award Recipient: JingYang
© 2022 The Authors
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2022-12-20
2024-05-09
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Canibacter zhuwentaonis sp. nov. and Canibacter zhoujuaniae sp. nov., isolated from Marmota himalayana
Int J Syst Evol Microbiol 72, 005633 (2023); https://doi.org/10.1099/ijsem.0.005633
/content/journal/ijsem/10.1099/ijsem.0.005633
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