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Volume 72, Issue 12

sp. nov., a moderately thermophilic bacterium isolated from hot spring microbial mat No Access

Abstract

Two closely related, aerobic, Gram-stain-negative, motile, oval-shaped, non-endospore-forming, moderately thermophilic bacteria, designated strains SYSU G05006 and SYSU G05005, were isolated from a bioreactor enrichment and the original sample was collected from Rehai National Park, Tengchong, Yunnan Province, PR China. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that these two strains were closely related to YIM 72346 (96.75 and 96.89 % sequence similarity, respectively). The whole genome size of strain SYSU G05006 was 3.87 Mbp with a DNA G+C content of 75.33 mol%. The average nucleotide identity (ANI based on the MUMmer algorithm≤90.31 % and ANI based on ≤89.36 %) and digital DNA–DNA hybridization (≤35.10 %) values between strain SYSU G05006 and other members of the family were all lower than the threshold values recommended for distinguishing novel prokaryotic species. Optimal growth of the strain was observed at 55 °C and pH 6.0. Ubiquinone-10 was the predominant respiratory lipoquinone. The major cellular fatty acids included iso-C, C ω5, summed feature 5 and summed feature 7. The major polar lipids consisted of diphosphatidylglycerol, one unidentified aminolipid, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified phospholipids and two unidentified lipids. Based on results of phylogenetic analyses, comparative genomics and phenotypic characteristics, we describe a new species of the genus represented by strain SYSU G05006 (=KCTC 82831=MCCC 1K06125), for which we propose the name sp. nov.

  • Received:
  • Accepted:
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Keyword(s): bioreactor enrichment , Caldovatus aquaticus sp. nov. , hot spring microbial mat and polyphasic taxonomy
Funding
This study was supported by the:
  • Natural Science Foundation of Guangdong Province - General Project (Award 2022A1515011673)
    • Principle Award Recipient: YeTian
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: Jian-YuJiao
  • National Natural Science Foundation of China (Award 32100042)
    • Principle Award Recipient: Wen-DongXian
  • National Natural Science Foundation of China (Award 32170101)
    • Principle Award Recipient: ShaTan
  • National Natural Science Foundation of China (Award 91951205)
    • Principle Award Recipient: Wen-JunLi
© 2022 The Authors
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2022-12-07
2024-04-26
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Caldovatus aquaticus sp. nov., a moderately thermophilic bacterium isolated from hot spring microbial mat
Int J Syst Evol Microbiol 72, 005627 (2023); https://doi.org/10.1099/ijsem.0.005627
/content/journal/ijsem/10.1099/ijsem.0.005627
/content/journal/ijsem/10.1099/ijsem.0.005627
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