1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 10

A novel species of lactic acid bacteria, sp. nov., isolated from alfalfa silage Open Access

Abstract

In this study, we isolated a novel strain of lactic acid bacteria, AF129, from alfalfa silage prepared locally in Morioka, Iwate, Japan. Polyphasic taxonomy was used to characterize the bacterial strain. The bacterium was rod-shaped, Gram-stain-positive, non-spore-forming and catalase-negative. The strain grew at various temperatures (15–40°C) and pH levels (4.0–8.0). The optimum growth conditions were a temperature of 30°C and a pH of 6.0. AF129 exhibited growth at salt (NaCl) concentrations of up to 6.5 % (w/v). The G+C content of the strain’s genomic DNA was 41.5 %. The major fatty acids were C, Cω9, Ccyclo ω8 and summed feature 8. 16S rRNA gene sequencing revealed that AF129 represents a member of the genus and it has higher sequence similarities with (98.4 %), (97.5 %) and (97.4 %). The digital DNA–DNA hybridization values for AF129 and phylogenetically related species of the genus ranged from 19.8% to 24.1%. The average nucleotide identity of the strain with its closely related taxa was lower than the threshold (95 %–96 %) used for species differentiation. In the light of the above-mentioned physiological, genotypic, chemotaxonomic and phylogenetic evidence, we confirm that AF129 represents a member of the genus and constitutes a novel species; we propose the name sp. nov. for this species. The type strain is AF129 =MAFF 518002 =JCM 34518=BCRC 81335.

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  • Accepted:
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Keyword(s): alfalfa silage , Bacillota , lactic acid bacteria and Ligilactobacillus pabuli
Funding
This study was supported by the:
  • NARO GenBank Project (Microorganism Section)
    • Principle Award Recipient: MasanoriTohno
  • NARO Gender Equality Program
    • Principle Award Recipient: HisamiKobayashi
  • NARO Gender Equality Program
    • Principle Award Recipient: MasanoriTohno
  • JSPS KAKENHI (Award 20K06384)
    • Principle Award Recipient: MasanoriTohno
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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A novel species of lactic acid bacteria, Ligilactobacillus pabuli sp. nov., isolated from alfalfa silage
Int J Syst Evol Microbiol 72, 005587 (2022); https://doi.org/10.1099/ijsem.0.005587
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