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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 10

sp. nov., a novel gut bacterium isolated from a Yellow Sea oyster No Access

Abstract

A Gram-stain-negative, oxidase- and catalase-positive, facultative anaerobic motile bacterium, designated strain OG9-811, was isolated from the gut of an oyster collected in the Yellow Sea, Republic of Korea. The strain grew at 10–37 °C, pH 6.0–9.0 and with 0.5–10% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain OG9-811 affiliated with the genus , with the highest sequence similarity of 98.2% to ATCC BAA-450 followed by R-40492 (98.0 %), LMG 20362 (97.7 %) and LMG 20536 (97.6 %); other relatives were JCM 16456 (97.4 %), NBRC 103150 (97.0 %) and CIP 102972 (97.0 %). The complete genome of strain OG9-811 comprised two chromosomes of a total 4 807 684 bp and the G+C content was 50.2 %. Results of analysis based on the whole genome sequence showed the distinctiveness of strain OG9-811. The average nucleotide identity (ANI) values between strain OG9-811 and the closest strains ATCC BAA-450, R-40492, LMG 20362, KCTC 12702 JCM 16456, ATCC 33809 and CIP 102972 were 73.0, 72.6, 73.3, 73.0, 72.7, 78.5 and 77.8 %, respectively, while the digital DNA–DNA hybridization values between strain OG9-811 and the above closely related strains were 20.8, 21.2, 20.8, 21.7, 20.7, 23.2 and 22.4 %, respectively. The major fatty acids of strain OG9-811 were summed feature 3 (C 7 and/or C 6), summed feature 8 (C c and/or C 7) and C. The polar lipids contained phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Strain OG9-811 contained Q-8 as a quinone. On the basis of polyphasic taxonomic characteristics, strain OG9-811 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is OG9-811 (=KCTC 72623=GDMCC 1.2610).

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Keyword(s): gut of oyster , polyphasic taxonomy and proteobacteria
© 2022 The Authors
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2022-10-21
2024-05-04
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Vibrio ostreae sp. nov., a novel gut bacterium isolated from a Yellow Sea oyster
Int J Syst Evol Microbiol 72, 005586 (2022); https://doi.org/10.1099/ijsem.0.005586
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