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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 72, Issue 10

sp. nov. and sp. nov. isolated from an aquaculture farm No Access

Abstract

The three novel bacterial strains designated as 3Y2, 4Y16 and 4Y11 were isolated from an aquaculture farm and characterized using a polyphasic taxonomic approach. These strains were determined to be catalase- and oxidase-positive and to hydrolyze gelatin and aesculin. The results of 16S rRNA gene-based phylogenetic analysis indicated that the three strains were related to members of the genus . The phylogenomic results further indicated that the three strains formed two independent branches distinct from reference type strains within this genus. The digital DNA–DNA hybridization (dDDH), average nucleotide identity (ANI) and average amino acid identity (AAI) values between the three strains and their relatives were far below the thresholds of 70 % dDDH, 95–96 % ANI and 95 % AAI for species definition, respectively, indicating that the three strains represent two novel genospecies. The results of chemotaxonomic characterization indicated that the major cellular fatty acids of the three strains were summed feature 3 (Cω6 and/or C ) and C; the common main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol; the respiratory quinone was ubiquinone-8. The genomic DNA G+C contents of the three strains were 70.2, 70.1 and 69.7%, respectively. On the basis of the different genotypes and distinctive phenotypes such as the phosphatidylcholine and glycolipid only in 3Y2 and the utilization of malic acid and trisodium citrate only in 4Y11, strains 3Y2 and 4Y11 are concluded to represent two novel species of the genus , for which the names sp. nov. (type strain 3Y2 = GDMCC 1.2584 = KCTC 82813) and sp. nov. (type strain 4Y11 = GDMCC 1.1935 = JCM 34285) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome analysis , Ideonella alba , Ideonella aquatica , novel species and polyphasic taxonomy
Funding
This study was supported by the:
  • Science and Technology Program of Guangdong Province (Award 2021B1212050022)
    • Principle Award Recipient: HonghuiZhu
  • GDAS' Project of Science and Technology Development (Award 2020GDASYL-20200103028)
    • Principle Award Recipient: JuanDu
© 2022 The Authors
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2022-10-20
2025-01-21
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Ideonella alba sp. nov. and Ideonella aquatica sp. nov. isolated from an aquaculture farm
Int J Syst Evol Microbiol 72, 005578 (2022); https://doi.org/10.1099/ijsem.0.005578
/content/journal/ijsem/10.1099/ijsem.0.005578
/content/journal/ijsem/10.1099/ijsem.0.005578
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