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Abstract

An aerobic denitrifying bacterium, designated as strain CPY4, was isolated from activated sludge treating urban sewage under alternating aerobic/anaerobic conditions by an enrichment culture technique. Cells of strain CPY4 were Gram-stain-negative, aerobic, long rod-shaped, motile by means of single polar flagellum and capable of aerobic denitrification with citrate as the carbon source. Growth of strain CPY4 was observed at 10–45 °C (optimum, 30–35 °C), at pH 6.0–10.5 (optimum, pH 8.0–8.5) and in 0–5 % NaCl (optimum, 0–3 %; w/v). The 16S rRNA gene sequence of strain CPY4 showed the highest similarity to ZD1 (97.9 %), followed by 59N8 (97.6 %), JC2671 (97.2 %), ZT1 (97.1 %) and 102-Py4 (96.3 %). Genome comparisons between CPY4 and other species showed highest digital DNA–DNA hybridization with ZD1 (43.8 %) and highest average nucleotide identity (ANIb and ANIm) of genome nucleotide sequences with ZD1(90.7 and 92 %, respectively). Phylogenetic analysis revealed that strain CPY4 fell within the clade comprising the type strains of species and formed a phyletic line with them, which was distinct from other members of the family . The sole respiratory ubiquinone was quinone 8. The predominant fatty acids (>10 % of the total fatty acids) of strain CPY4 were summed feature 8 (C 6 and/or C 7), summed feature 3 (C 6 and/or C 7) and C. The genomic DNA G+C content was 62.7 mol %. In the polar lipid profile, diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl ethanolamine, phospholipids and aminolipids were the major compounds. Based on the genotypic and phenotypic data, strain CPY4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CPY4 (=JCM 34456=CGMCC 1.18722).

Funding
This study was supported by the:
  • FJIRSM and IUE Joint Research Fund (Award RHZX-2019-005)
    • Principle Award Recipient: YuChang-Ping
  • STS Project of Fujian-CAS (Award 2021T3014)
    • Principle Award Recipient: LiJiangwei
  • National Natural Science Foundation of China (Award 31870475)
    • Principle Award Recipient: HuAnyi
  • National Natural Science Foundation of China (Award 31500420)
    • Principle Award Recipient: LiJiangwei
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2022-11-11
2024-04-25
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