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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 10

sp. nov., isolated from L. ‘Cheungsam’ No Access

Abstract

A Gram-negative, aerobic, rod-shaped bacterium, designated DM2-R-LB4 was isolated from L. ‘Cheungsam’ in Andong, Republic of Korea. The strain DM2-R-LB4 grew at temperatures of 15–45 °C (optimum, 30–37 °C), pH of 5.5–9 (optimum, 8.0), and 0–2 % (w/v) NaCl concentration (optimum, 0%). Phylogenetic analyses based on the 16S rRNA gene sequences revealed that strain DM2-R-LB4 is related to species of the genus , and shared 97.8 and 97.5% similarity to KCTC 42244 and DSM 4733, respectively. The DNA G+C content was 67.9 mol% and genome analysis of the strain DM2-R-LB4 revealed that the genome size was 4 386 171 bp and contained 4 009 predicted protein-coding genes. The average nucleotide identity (ANI) values between strain DM2-R-LB4 and KCTC 42244, and DSM 4733 was 76.8 and 76.7 %, respectively, while the values of digital DNA–DNA hybridization (dDDH) were 20.7 and 20.6 %, respectively. C 2-OH, C, and summed feature 8 (C ω6c and/or C ω7c) were the major fatty acids (>10 %) in the strain DM2-R-LB4. The polar lipids comprised diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), sphingoglycolipid (SGL), glycolipid (GL), phospholipid (PL), and two unidentified polar lipids (L1 and L2). Ubiquinone-10 (Q-10) was the only respiratory quinone. The polyamine pattern was found to contain homospermidine, putrescine, and spermidine. The results of phylogenetic anlayses, polyphasic studies, revealed that strain DM2-R-LB4 represents a novel species of the genus , for which the name sp. nov., is proposed. The type strain is DM2-R-LB4 (=KCTC 92075 = GDMCC 1.3018).

  • Received:
  • Accepted:
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Keyword(s): average nucleotide identity , Cannabis sativa , endophytes , novel species and Sphingomonas
Funding
This study was supported by the:
  • Korea Research Institute of Bioscience and Biotechnology (Award KGM5382212)
    • Principle Award Recipient: Cheol JeongJae
  • the Basic Science Research Program through the National Research Foundation of Korea (NRF) (Award 2020R111A2072308)
    • Principle Award Recipient: LeeJiyoung
© 2022 The Authors
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2022-10-19
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Sphingomonas cannabina sp. nov., isolated from Cannabis sativa L. ‘Cheungsam’
Int J Syst Evol Microbiol 72, 005566 (2022); https://doi.org/10.1099/ijsem.0.005566
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