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Volume 72, Issue 11

sp nov. and sp nov., isolated from ornamental crops in California Open Access

Abstract

Five bacterial strains were isolated from symptomatic leaves of , sp. and sp. in California. Colonies isolated on King’s medium B (KMB) appeared white, mucoid and round, similar to species. Phylogenetic analyses based on 16S rRNA, , and genes placed the bacteria into three distinct groups within that were most closely related to , or . To further characterize the strains, phenotypic analyses and the following tests were performed: fatty acid methyl ester composition, LOPAT, fluorescence on KMB, Biolog assay, and transmission electron microscopy. Finally, whole genome sequencing of the strains was conducted, and the sequences were compared with reference genomes of species based on average nucleotide identity (ANI). The first group, which consists of three strains isolated from delphinium, hydrangea and achillea, had 95.6–96.9 % pairwise ANI between each other; the second group consists of two strains isolated from delphinium that had 100 % pairwise ANI. Although comparisons of the two groups with publicly available genomes revealed closest relationships with (91.6 %), (88.3 %) and (86.7 %), ANI values were less than 95 % compared to all validly published pseudomonads. Combining genomic and phenotypic data, we conclude that these strains represent two new species and the names proposed are sp. nov. (type strain DSMZ 11 30 42=LMG 32 434) for the strains isolated from delphinium, achillea and hydrangea and sp. nov. (DSMZ 11 30 43=LMG 32 432) for the two strains isolated from delphinium. The specific epithets and were selected based on the close phylogenetic relationship of strains with and on the geographic location of isolation, respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bacterial taxonomy , ornamentals and Pseudomonas
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-11-02
2024-05-04
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Pseudomonas californiensis sp. nov. and Pseudomonas quasicaspiana sp. nov., isolated from ornamental crops in California
Int J Syst Evol Microbiol 72, 005565 (2022); https://doi.org/10.1099/ijsem.0.005565
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