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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 10

sp. nov., sp. nov. and sp. nov., isolated from salt lakes on the Tibetan Plateau No Access

Abstract

Four Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and non-motile strains (CAK1W, CAK8W, CAK57W and CCL10W) were isolated from salt lakes in China. Comparisons based on the 16S rRNA gene sequences showed that the four strains show less than 98.9% similarity to species of the genus . The phylogenetic tree reconstructed based on 16S rRNA gene sequences also showed that species are the most closely related neighbours of the four strains. The sequenced draft genome sizes of strains CAK1W, CAK8W, CAK57W and CCL10W were 3.01, 2.95, 3.01 and 3.04 Mbp with G+C contents of 37.3, 35.8, 37.5 and 36.6 %, respectively. The phylogenomic trees reconstructed based on the UBCG and GET_PHYLOMARKERS pipelines all demonstrated that the four strains belong to the genus . The calculated pairwise orthologous average nucleotide identity based on usearch, digital DNA–DNA hybridization and average amino acid sequence identity values among strains CAK1W, CAK8W, CAK57W, CCL10W and other species of the genus were equal or lower than 91.1, 43.5 and 92.2%; the values between strains CAK1W and CAK57W were 98.8, 90.2 and 99.0 %, respectively. The respiratory quinone of the four strains was MK-6. Their major fatty acids were iso-C, C 10, iso-C and anteiso-C. The major polar lipids of the four strains included phosphatidylethanolamine, an unidentified aminolipid and two kinds of unidentified lipids, and only strain CCL10W contained diphosphatidylglycerol. Based on the above descriptions, strains CAK1W, CAK8W, CAK57W and CCL10W should belong to the genus and represent three independent novel species, for which the names sp. nov. (type strain CAK1W=GDMCC 1.2644=KCTC 82857), sp. nov. (type strain CAK8W=GDMCC 1.2646=KCTC 82859) and sp. nov. (type strain CCL10W=GDMCC 1.2631=KCTC 82860) are proposed.

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Keyword(s): Psychroflexus , salt lake and Tibetan Plateau
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2022-10-21
2024-05-06
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Psychroflexus curvus sp. nov., Psychroflexus longus sp. nov. and Psychroflexus montanilacus sp. nov., isolated from salt lakes on the Tibetan Plateau
Int J Syst Evol Microbiol 72, 005563 (2022); https://doi.org/10.1099/ijsem.0.005563
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