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Volume 72, Issue 10

sp. nov., isolated from river sediment No Access

Abstract

A Gram-stain-negative, yellow-pigmented, motile, flagellated and rod-shaped bacterium, designated as 13A, was isolated from a river sediment sample of Fuyang River in Hengshui City, Hebei Province, PR China. Strain 13A grew at 10–37 °C (optimum, 30 °C), at pH 5.0–11.0 (optimum, pH 7.0) and at 0–7 % (w/v) NaCl concentration (optimum, 0 %). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain 13A belongs to the genus , and was most closely related to DSM 21749 (97.8 %), DSM 16239 (97.5 %), GIM 1.690 (97.3 %) and CGMCC 1.10752 (96.9 %). Meanwhile, the type species ATCC 29487 was selected as a reference strain (95.2 %). The genomic size of strain 13A was 3.0 Mb and the DNA G+C content was 69.0 %. The average nucleotide identity values between strain 13A and each of the reference type strains DSM 21749, DSM 16239, GIM 1.690, CGMCC 1.10752 and ATCC 29487 were 75.9, 76.1, 77.7, 78.0 and 73.2 %, respectively. The digital DNA–DNA hybridization values between strain 13A and each of the reference type strains were 21.7, 22.2, 21.9, 22.7 and 23.2 %, respectively. The average amino acid identity values between strain 13A and each of the reference type strains were 72.5, 72.9, 72.3, 75.0 and 69.2 %, respectively. The major fatty acids were iso-C, iso-C and summed feature 9 (iso-C 9 and/or C 10-methyl). The sole respiratory quinone was identified as ubiquinone-8. The polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified lipid, four unidentified phospholipids and two unidentified glycolipids. Based on the phenotypic, physiological, phylogenetic and chemotaxonomic data, strain 13A represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 13A (=JCM 34786=GDMCC 1.2722).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome , Lysobacter selenitireducens sp. nov. , phylogenomic tree and Venn diagram
Funding
This study was supported by the:
  • Natural Science Research Project of Hengshui University (Award 2018ZX001)
    • Principle Award Recipient: ShuzhenWei
  • Major Program of Shandong Province Natural Science Foundation (Award ZR2020ZD19)
    • Principle Award Recipient: AijvLiu
  • Natural Science Foundation of Shandong Province (Award ZR2020MD121)
    • Principle Award Recipient: HongliangLiu
  • National Natural Science Foundation of China (Award 42177403)
    • Principle Award Recipient: HongliangLiu
© 2022 The Authors
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2022-10-19
2024-05-18
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Lysobacter selenitireducens sp. nov., isolated from river sediment
Int J Syst Evol Microbiol 72, 005550 (2022); https://doi.org/10.1099/ijsem.0.005550
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